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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2011.tde-30052012-085711
Document
Author
Full name
Gustavo Yuzo Ujikawa
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Troncone, Lanfranco Ranieri Paolo (President)
Franco, Marcelo de
Rocha, Adriana Rios Lopes
Title in Portuguese
Estudo da susceptibilidade de camundongos bons ou maus respondedores para inflamação aguda a neurotoxinas empregadas em modelos de Parkinson.
Keywords in Portuguese
Camundongos
Doença de Parkinson
Hidroxilase
Imunohistologia
Inflamação
Lesões em animais
Abstract in Portuguese
Estudos apontam a neuroinflamação como um dos possíveis agravantes do mal de Parkinson. Aqui investigamos a susceptibilidade de duas linhagens de camundongos selecionados quanto a suas capacidades de produzir alta (AIRmax) ou baixa (AIRmin) resposta inflamatória aguda aos agentes neurotóxicos 1-Metil-4-Fenil-1,2,3,6-Tetrahidropirimidina (MPTP) (Tratamento agudo: 4 x 20 mg/kg ou 5 x 20 mg/kg s.c. a cada 2 horas em um único dia) e rotenona (tratamento crônico: infusão s.c. contínua por bomba osmótica Alzet a 3, 6 e 12 mg/kg/dia por 28 dias ou sub-crônico: injeção i.p na dose de 3 mg/kg/dia durante 10 dias). A avaliação motora contou com Rotarod empregando paradigma de 5 minutos e rotação crescente de 5 a 50 rpm. A lesão foi quantificada por imunohistoquímica para tirosina hidroxilase em cortes de estriado e substância negra. Os resultados sugerem que ambas as linhagens AIRmax e AIRmin, são resistentes a lesão neuronal por MPTP ou rotenona. Conjecturamos que as linhagens se diferenciam quanto a resposta inflamatória periférica, mas não de origem central (neuroinflamação).
Title in English
Susceptibility of mice genetically selected for acute inflammatory reactions to neurotoxins used in Parkinson´s disease models.
Keywords in English
Hydroxylase
Immunohistology
Inflammation
Lesions in animals
Mice
Parkinson's disease
Abstract in English
Studies suggest that neuroinflammatory processes are involved in Parkinson's disease. Here we investigated the susceptibility of two inbred strains of mice selected for their ability to produce high (AIRmax) or low (AIRmin) acute inflammatory response to neurotoxins 1-Methyl-4-phenyl-1,2,3,6-Tetrahydropyridine (MPTP) (4 x 20 mg/kg or 5 x 20 mg/kg s.c. every 2 hours in a single day) and rotenone (chronic treatment: continuous s.c. infusion of 3, 6 and 12 mg/kg/day by Alzet osmotic pump for 28 days or sub-chronic treatment: i.p. injection at a dose of 3 mg/kg/day for 10 days). The animals were evaluated by rotarod apparatus in sessions of 5 minutes and constantly increasing speed from 5 to 50 rpm. The lesion was quantified by tyrosine hydroxylase immunohistochemistry in sections of striatum and substantia nigra. The results suggest that both strains AIRmax and AIRmin are resistant to neuronal damage by MPTP or rotenone. We conjecture that the strains differ concerning the peripheral inflammatory response, but not in neuroinflammatory mechanisms.
 
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Publishing Date
2012-07-23
 
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