• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2009.tde-29042010-095050
Document
Author
Full name
Tatiana Sayuri Matsuda
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Gomez, José Gregorio Cabrera (President)
Taciro, Marilda Keico
Vicente, Elisabete Jose
Title in Portuguese
Isolamento de bactéria produtoras de polihidroxialcanoatos de cadeia curta e média a partir de óleos vegetais
Keywords in Portuguese
Ácido láurico
Aeromonas
Óleo de soja
P3HB-co-3HHx
Polihidroxialcanoatos
Abstract in Portuguese
O potencial de bactérias em produzir polihidroxialcanoatos (PHA) a partir de óleo de soja foi avaliado com ênfase em Aeromonas spp. Dez isolados apresentando características de Aeromonas spp. (colônias amarelas em GSP agar e produção de P3HB-co-3HHx) e perfis ARDRA similares foram obtidos. Dois isolados produzindo PHAMCL foram também obtidos e experimentos de identificação adicionais serão necessários para confirmar se pertencem ao gênero Aeromonas. P3HB-co-3HHx contendo diferentes frações molares de 3HHx foram produzidos. Isolados produzindo P3HB-co-3HHx a partir de óleo de soja ou ácido láurico foram incapazes de produzir P3HB a partir de glicose sugerindo sua incapacidade de gerar monômeros 3HB a partir de acetil-CoA. Introdução de plasmídeo abrigando genes de biossíntese de P3HB de Ralstonia eutropha em isolados capazes de utilizar eficientemente óleo de soja como fonte de carbono permitiu a produção de P3HB, mas não PHA contendo monômeros de cadeia média, sugerindo uma transferência ineficiente de intermediários a partir da β-oxidação para PHA sintase.
Title in English
Isolation of bactéria producing polyhydroxyalkanoates containing short-chain-length and medium-chain-length monomers from plant oils
Keywords in English
Aeromonas
Lauric acid
P3HB-co-3HHx
Polyhydroxyalkanoates
Soybean
Abstract in English
The potential of polyhydroxyalknoates (PHA) production from plant oils was evaluated especially in Aeromonas spp. Ten isolates presenting features of Aeromonas spp. (yellow colonies in GSP agar and production of P3HB-co-3HHx) and similar ARDRA profiles were obtained. Two isolates producing PHAMCL were also obtained and further identification experiments will be needed to confirm their position in Aeromonas genus. P3HB-co-3HHx containing different molar fractions of 3HHx were produced. Isolates producing P3HB-co-3HHx from soybean oil or lauric acid were unable to produce P3HB from glucose suggesting their incapability to generate 3HB monomers from acetyl-CoA. Introduction of a plasmid harboring P3HB biosynthesis genes from Ralstonia eutropha into isolates able to use efficiently soybean oil as carbon source allowed the production of P3HB but not PHA containing HAMCL suggesting an inefficient transfer of intermediates from β-oxidation to PHA synthase.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2010-05-19
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2024. All rights reserved.