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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2009.tde-29042010-092836
Document
Author
Full name
Mauricio Rocha Dimitrov
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Lemos, Eliana Gertrudes Macedo (President)
Spira, Beny
Vicente, Elisabete Jose
Title in Portuguese
Construção de biblioteca metagenômica e prospecção de genes para a síntese de polihidroxalcanoatos
Keywords in Portuguese
Bibliotecas metagenômicas
Biotecnologia
Fosmídeo
PHA sintase
Polihidroxialcanoatos
Polímeros bacterianos
Abstract in Portuguese
Os microrganismos constituem dois terços da diversidade biológica na Terra, no entanto, muitos deles não podem ser cultivados por técnicas tradicionais. Portanto, o acesso a esta diversidade tem sido feita através da utilização de técnicas independentes de cultivo. Diante deste panorama, a metagenômica apresenta-se como uma alternativa, pois dispensa a necessidade de cultivo. Tal técnica possibilita inclusive a identificação e utilização do potencial metabólico destes organismos para o desenvolvimento de novos processos e produtos. Os polihidroxialcanoatos (PHAs) são poliésteres bacterianos, acumulados intracelularmente em forma de grânulos, cujas propriedades são similares a de alguns plásticos de origem petroquímica. O objetivo deste trabalho foi identificar e avaliar a diversidade de genes relacionados à produção de PHAs em bibliotecas metagenômicas de solo. A prospecção realizada resultou na identificação de clones contendo o gene phaC. De uma forma geral, pôde-se concluir que ainda há uma grande diversidade deste gene a ser descoberta no ambiente estudado.
Title in English
Metagenomic library construction for PHA synthase screening
Keywords in English
Bacterial polyesters
Biotechnology
Fosmid
Metagenomic Library
PHA synthase
Polyhydroxyalkanoates
Abstract in English
Microorganisms constitute two third of the Earth's biological diversity, however, many of them cannot be cultured by standard techniques. Therefore, access to this diversity has been achieved through the use of culture-independent techniques. Facing this scenario, the metagenomic presents itself as an alternative, since it eliminates the need for cultivation. This technique also allows the identification and use of the metabolic pathways of these organisms to develop new processes and products. The polyhydroxyalkanoates (PHAs) are bacterial polyesters accumulated as granules, whose properties are similar to some plastics of petrochemical origin. The aim of this work was to identify and access the diversity of genes related to PHAs production in soil metagenomic libraries. The screening resulted in the identification of clones containing the phaC gene. In a general way, it was concluded that there is still a considerable diversity of this gene to be discovered in the study environment.
 
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Publishing Date
2010-05-19
 
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