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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2017.tde-28062017-140543
Document
Author
Full name
Nayara Gomes Luiz Santos
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Astray, Renato Mancini (President)
Prudencio, Carlos Roberto
Ruiz, Rita de Cássia
Title in Portuguese
Desenvolvimento de um método de imunofluorescência aplicado à detecção de anticorpos contra o arbovírus Mayaro.
Keywords in Portuguese
Células S2
Expressão de proteína
Imunofluorescência
Vírus Mayaro
Abstract in Portuguese
O vírus Mayaro (MAYV) é um Alphavirus artritogênico responsável por causar uma doença febril aguda com sintomas parecidos aos de Dengue não-hemorrágica, porém com o agravante, como a febre Chikungunya, de ocorrência de artralgia. Os dados epidemiológicos disponíveis ainda são poucos devido à falta de diagnóstico adequado, pois algumas das técnicas desenvolvidas apresentam dificuldades quanto à coleta de amostra, devido à curta viremia, e ao background que interfere na interpretação dos resultados, subestimando o real número dos casos de infecção. Isso é preocupante principalmente em tempos de co-circulação de outras arboviroses como Dengue, Zika e Chikungunya. Neste trabalho desenvolvemos um método de imunofluorescência indireta dirigido à detecção de anticorpos contra a glicoproteína E2 do vírus Mayaro expressa em células S2 de Drosophila melanogaster.
Title in English
Development of an immunofluorescence method applied to detection of antibodies against Mayaro arbovirus.
Keywords in English
Mayaro Virus
Immunofluorescence
Protein expression
S2 Cells
Abstract in English
Mayaro virus (MAYV) is an arthritogenic Alphavirus responsible for causing an acute febrile illness with symptoms similar to non-hemorrhagic Dengue, but with the aggravation, as Chikungunya fever, to develop arthralgia. The epidemiological data available still are few due to lack of proper diagnosis, because some of the techniques developed present difficulties regarding sample collection, due to the short viremia, and background that interferes with the interpretation of the results, underestimating the actual number of cases. This is a concern especially in periods of co-circulation of many other socioeconomic impact arboviruses, such as Dengue, Zika and Chikungunya. In this work we developed an indirect immunofluorescence method to the detection of antibodies against Mayaro E2 glycoprotein expressed in Drosophila melanogaster S2 cells.
 
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Publishing Date
2017-06-28
 
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