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Master's Dissertation
DOI
10.11606/D.87.2011.tde-24032011-170215
Document
Author
Full name
Alexandre Kopte Garcia
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Sette, Lara Durães (President)
Destefano, Suzete Aparecida Lanza
Santos, Rafaella Costa Bonugli
Title in Portuguese
Avaliação da atividade lipolítica de fungos filamentosos da costa brasileira.
Keywords in Portuguese
Biorremediação
Biotecnologia
Emulsificantes
Enzimas
Fungos
Lipase
Abstract in Portuguese
A produção de compostos biologicamente ativos por micro-organismos marinhos tem atraído crescente interesse de biotecnólogos e microbiologistas nas últimas décadas. No presente trabalho 162 isolados de fungos filamentosos recuperados de amostras de água do mar e de dez diferentes macro-organismos marinhos foram avaliados quanto a produção de lipases pelo método de High Throughput Screening (HTS). Destes, 45 isolados tiveram a produção de lipases confirmada individualmente. A atividade lipolítica foi quantificada, e os resultados sugerem que fungos filamentosos oriundos de ambientes marinhos tem maior potencial para produção de lipases estáveis em pH alcalino. Entre estes, Fusarium sp. CBMAI 1227, Aspergillus parasiticus CBMAI 1228 e Trichoderma sp. CBMAI 1229 apresentaram atividade expressiva e de interesse biotecnológico em pH 8,0 (23,1, 12,7 e 12,2 U respectivamente). Os resultados deste trabalho demonstram o potencial dos fungos provenientes de ambiente marinho para aplicações biotecnológicas, e estimulam novos estudos envolvendo lipases.
Title in English
Evaluation of the lipolytic activity of filamentous fungi from the brazilian coast.
Keywords in English
Bioremediation
Biotechnology
Emulsifiers
Enzymes
Fungi
Lipase
Abstract in English
The production of biologically active compounds by marine microorganisms has been drawing increasing interest of biotechnologists and microbiologists in the last decades. In the present work 162 filamentous fungi isolates recovered from water samples and ten different marine macroorganisms were evaluated for the production of lipases by the method of High Throughput Screening (HTS). Out of those, 45 had the lipase production individually confirmed. The lipolytic activity was quantified, and the results suggest that marine-derived filamentous fungi have a greater potential for the production of lipases stable under alkaline pH. Among these, Fusarium sp. CBMAI 1227, Aspergillus parasiticus CBMAI 1228 and Trichoderma sp. CBMAI 1229 showed expressive and biologically interesting activity in pH 8,0 (23,1, 12,7 and 12,2 U/min respectively). The results of the present work show the potential of marine-derived fungi in biotechnological applications, and stimulate new studies involving lipases.
 
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Publishing Date
2011-08-16
 
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