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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2010.tde-23082010-154523
Document
Author
Full name
Patricia Rossi do Sacramento
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Botosso, Viviane Fongaro (President)
Durigon, Edison Luiz
Harsi, Charlotte Marianna
Title in Portuguese
Diversidade genética da neuraminidase de vírus Influenza A, isolados de crianças internadas na cidade de São Paulo, de 1995 a 2006.
Keywords in Portuguese
Biodiversidade
Diversidade genética
Epidemiologia molecular
Influenza
Neuraminidase
Reação de seqüenciamento
Reação em Cadeia por Polimerase
RT-PCR
Seqüenciamento genético
Vírus
Abstract in Portuguese
O presente estudo teve por objetivos caracterizar os vírus Influenza circulantes na cidade de São Paulo e verificar a variabilidade genética do gene da neuraminidase (NA) dos Influenzavírus A. Um total de 3009 amostras de crianças internadas no Hospital Universitário da USP, no período de 1995 a 2006, foram submetidas à duplex RT-PCR para detecção dos Influenzavírus A e B (IA e IB). As amostras positivas para IA foram submetidas à subtipagem pela multiplex RT-PCR. Cento e trinta e três amostras (4,4%) foram positivas, sendo 88,0% (117/133) IA e 12,0% (16/133) IB. Entre as amostras IA, 94 eram H3N2, 7 H1N1 e 16 não foram subtipadas pela multiplex. Um total de 74 amostras (71 H3N2 e 3 H1N1) tiveram o gene da neuraminidase sequenciado (total ou parcialmente). As sequências obtidas foram submetidas à análise filogenética, sendo verificado o agrupamento das cepas circulantes em clusters por ano de isolamento, demonstrando sua evolução temporal. Quando comparadas com as cepas vacinais utilizadas no período, foi verificada uma boa similaridade.
Title in English
Genetic diversity of Neuraminidase of Influenza A virus, isolated from children hospitalized in São Paulo city, from 1995 to 2006.
Keywords in English
Biodiversity
Genetic diversity
Genetic seqüencing
Influenza
Molecular epidemiology
Neuraminidase
Polymerase Chain Reaction
RT-PCR
Seqüencing reaction
Virus
Abstract in English
The aim of this study was to characterize Influenza virus circulating in São Paulo city and the neuraminidase (NA) gene sequence of Influenzavirus A in 3009 samples from children hospitalized at USP University Hospital, from 1995 to 2006. Samples underwent duplex RT-PCR for detection and typing of Influenza virus A and B (IA and IB) and also were submitted to multiplex PCR for subtyping of IA. Among 3009 samples, 4.4% were Influenza virus, being 88.0% IA (117/133) and 12.0% IB (16/133). Among samples of IA, 94 were characterized as H3N2, 7 H1N1 and 16 were not subtyped. A total of 74 samples (71 H3N2 and 3 H1N1) had the NA gene sequenced (total or partially). Sequences obtained were compared to sequences of the world and sequences of vaccine strains. Brazilian samples were grouped in clusters with samples isolated in the same year. It was also found a good similarity between circulating strains and vaccine strains.
 
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Publishing Date
2010-09-16
 
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