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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2011.tde-23032011-143651
Document
Author
Full name
Emy Tiyo Mano
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Araujo, Welington Luiz de (President)
Biondo, Ronaldo
Maldonado, Gabriel Padilla
Title in Portuguese
Identificação de genes de Burkholderia sp. associados ao controle biológico de Pectobacterium carotovora.
Keywords in Portuguese
Bactérias gram-negativas
Clonagem
Controle biológico vegetal
Enzimas pectinolíticas
Mutação genética
Podridão (doença da planta)
Abstract in Portuguese
A bacteria Pectobacterium carotovora causa danos a diferentes hospedeiros por meio da produção de enzimas pectinolíticas que degradam o pectato de cálcio da lamela media próximo a parede celular, causando extravasamento do conteúdo celular, sintomas da podridão mole. Bactérias do gênero Burkholderia tem se mostrado capazes em controlar a podridão mole em orquídeas, no entanto, os aspectos moleculares envolvidos neste controle ainda não foram estudados. Neste trabalho, foram avaliados 602 transformantes quanto a sua habilidade em inibir os sintomas da podridão mole, onde foram observados 16 mutantes defectivos no controle da doença. Destes, foram identificados sete diferentes genes inativados pelo transposon Tn5, e estes genes podem estar envolvidos em processos de síntese de aleloquímicos, competição por nutrientes, adaptação a condições ambientais, e na interação com o hospedeiro e/ou entre microrganismos. No entanto, o envolvimento destes genes na perda da capacidade em controlar a podridão mole deve ser melhor estudado.
Title in English
Identification of genes of Burkhoderia sp. associated with biological control of Pectobacterium carotovora.
Keywords in English
Biological control plant
Cloning
Gene mutation
Gram-negative bacteria
Pectic enzymes
Rot (plant disease)
Abstract in English
The bacterium Pectobacterium carotovora cause damage to different hosts and by production of pectic enzymes that degrade calcium pectate of the middle lamella near of the cell wall, causing overflow of cell content and consequently the soft rot. Burkholderia genus has proven able to control the soft rot in Orchids, however, the molecular aspects involved in the control have not been studied. In this work, 602 transformants were characterized for their ability to inhibit soft rot caused by P. carotovora. We identified 16 mutants showing shifts in inhibition pattern or lost of the ablitity to inhibit soft rot symptoms. Among these mutants, we identified 7 genes related to disease inhibition,and this genes may be involved in process of allelochemicals synthesis, competition for nutrients, adapting to environmental conditions, and interaction between the host and microorganisms. However, the involvement of these genes in loss of ability to control the soft rot disease is being further studied in details.
 
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EmyTMano_Mestrado.pdf (3.06 Mbytes)
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Publishing Date
2011-08-16
 
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