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Doctoral Thesis
DOI
https://doi.org/10.11606/T.87.2010.tde-22122010-132014
Document
Author
Full name
Felipe Alves Morais
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Botosso, Viviane Fongaro (President)
Ferreira, Antonio Walter
Figueiredo, Luiz Tadeu Moraes
Jerez, José Antonio
Mendonça, Ronaldo Zucatelli
Title in Portuguese
Ocorrência de anticorpos anti-hantavírus (IgG) em populações humanas na região Amazônica e no estado de São Paulo (Mata Atlântica), utilizando proteína recombinante (nucleocapsídio) do vírus Araraquara.
Keywords in Portuguese
Anticorpos
Antígeno recombinante brasileiro
Antígenos de vírus
ELISA
Epidemiologia
Hanta Vírus
HPS/FHSR
Infecções por Hantavírus
Soros (Diagnóstico)
Abstract in Portuguese
A hantavirose (infecção por Hantavírus) é uma das zoonoses que vem preocupando as autoridades sanitárias de todo o mundo. Sua ocorrência se deve principalmente os distúrbios ecológicos é transmitida ao homem através de inalação de partículas virais contida na excreta de roedores. São conhecidas duas doenças humanas distintas causadas pelo Hantavírus: a Febre Hemorrágica com Síndrome Renal (FHSR) e a Síndrome Pulmonar e Cardiovascular (SPCVH). O objetivo deste estudo foi verificar a ocorrência de anticorpos IgG anti-hantavírus, através do ELISA, em populações da Amazônia e Sudeste brasileiro, que vivem em contato com os roedores silvestres, utilizando a proteína recombinante do vírus Araraquara expressa em Escherichia coli. Do total de estudados 1308 soros humanos estudados, na Amazônia (1078) encontramos 59 soros positivos (5%). Na cidade Machadinho do OesteRO os soros coletados durante o ano 2003, foram analisados 638, onde foram encontrados 20 soros positivos (4,5%); e no Rio Machado RO. foram analisados 435 soros da população ribeirinha onde foram encontrados 39 (5%) soros positivos, respectivamente. Após análise realizada em 151 soros humanos provenientes do Vale do Ribeira, em 2007; e 84 no Pontal do Paranapanema, em 2008, foram observados 14 positivos (9%) e 6 (7%) das amostras, respectivamente.
Title in English
Detection of antibodies (IgG) against hantavirus in human population of Amazon region and the state of Sao Paulo (Atlantic Forest), using recombinant antigen of Araraquara virus.
Keywords in English
Antibodies
Brazilian recombinant antigen
ELISA
Epidemiology
Hanta Virus
Hantavirus Infections
HPS/FHSR
Soros (Diagnosis)
Virus antigens
Abstract in English
The genus Hantavirus of the family Bunyaviridae includes a large number of rodent-borne viruses that are distributed worldwide. The occurrence is due mainly to ecological disturbances and it is transmitted to the humans through inhalation of virus particles contained in the excreta of wild rodents. Two different human diseases known to be caused by Hantavirus: are Hemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Cardiopulmonary Syndrome (HPS). The main objective of this study was detected antibody against hanatavirus (IgG) by ELISA, in Amazon region and Brazilian Southwest populations who live in contact with the wild rodents, using recombinant protein (antigen) of the Araraquara virus expressed in Escherichia coli. We study 1308 human sera (1078 from Amazon region) and there were found 59 (5%) positive sera. From the city of Machadinho do Oeste RO (2003 year), 633 sera were analysed, where there were found to be 20 positive (4.5%) serums. In Machado river RO (2005 year), 435 sera of the river-dwelling population were analysed where there were found 39 (5%) positive sera, respectively. After analysis was accomplished for 151 human sera coming from the Vale do Ribeira - SP, in 2007, and 84 from the Pontal do Paranapanema - SP, in 2008, 14 (9%) and 6 (7%) of the samples were observed to be positive, respectively.
 
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Publishing Date
2011-01-17
 
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