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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2010.tde-22122010-095359
Document
Author
Full name
Patrícia Ferreira Lopes
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Oliveira, Valéria Maia de (President)
Araujo, Welington Luiz de
Vasconcellos, Suzan Pantaroto de
Title in Portuguese
Diversidade taxonômica e potencial de biodegradação de bactérias isoladas de reservatórios de petróleo da Bacia de Campos (RJ).
Keywords in Portuguese
Bactérias (Classificação)
Biodegradação
Biomarcadores do petróleo
Fingerprint genético
Genes RNAr 16S
Petróleo - Campos (RJ)
Reservatórios de petróleo
RNA (Genética)
Seqüenciamento genético
Abstract in Portuguese
O presente trabalho teve como objetivos caracterizar uma coleção de 98 bactérias isoladas de amostras de petróleo e água de formação de reservatórios da Bacia de Campos (RJ) utilizando técnicas de taxonomia molecular e avaliar o potencial de degradação de biomarcadores do petróleo. O sequenciamento e análise filogenética do gene RNAr 16S revelaram Bacillus firmus, megaterium, pumilus, sphaericus, simplex, cereus/B. thuringiensis Marinobacter lutaoensis, Halomonas shengliensis/H. alimentaria/ H.campisalis, Citreicella thiooxidans, Stenotrophomonas maltophilia, Achromobacter xylosoxidans, Micrococcus luteus, Kocuria rosea, Streptomyces alboniger/S. chartreusis /S. moderatus, Staphylococcus hominis e Staphylococcus pasteuri/S. warneri. Os resultados evidenciaram a preferência pela biotransformação do ácido nonadecanóico e esqualano. A caracterização da microbiota presente nos reservatórios e avaliação do potencial de biodegradação pode contribuir para fornecer subsídios para estudos futuros sobre os mecanismos biológicos responsáveis pela biodegradação do petróleo.
Title in English
Taxonomic diversity and biodegradation potential of bacteria isolated from oil reservoirs of the Campos Basin (RJ).
Keywords in English
16S rRNA genes
Bacteria (Rank)
Biodegradation
Genetic fingerprint
Genetic sequencing
Oil -Campos (RJ)
Petroleum biomarkers
Petroleum reservoirs
RNA (Genetics)
Abstract in English
This study is aimed to characterize a collection of 98 bacteria isolated from oil and formation water samples derived from reservoirs of the Campos Basin (RJ) using molecular biology-based techniques and to evaluate the degradation potential of petroleum biomarkers. Further sequencing and phylogenetic analysis of 16S rRNA genes revealed species of Bacillus firmus, megaterium, pumilus, sphaericus, simplex, cereus/thuringiensis, Marinobacter lutaoensis, Halomonas shengliensis/H. alimentaria/H. campisalis, Citreicella thiooxidans, Stenotrophomonas maltophilia, Achromobacter xylosoxidans, Micrococcus luteus, Kocuria rosea, Streptomyces alboniger/S. chartreusis/S. moderatus, Staphylococcus hominis and Staphylococcus pasteuri/S. warneri. The results showed the preference of bacteria for the biotransformation of nonadecanoic acid and squalane. The characterization of the microbiota associated to reservoirs and the evaluation of their biodegradation potential may provide subsidies for future studies about the biological mechanisms responsible for petroleum biodegradation.
 
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Publishing Date
2011-02-03
 
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