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Master's Dissertation
DOI
https://doi.org/10.11606/D.87.2018.tde-22102018-154848
Document
Author
Full name
Wesley Leoricy Marques
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Gombert, Andreas Karoly (President)
Azzoni, Adriano Rodrigues
Barros, Mário Henrique de
Title in Portuguese
Engenharia metabólica de Saccharomyces cerevisiae para o aumento do rendimento energético do metabolismo da sacarose.
Keywords in Portuguese
Saccharomyces cerevisiae
Bioenergética
Engenharia evolutiva
Engenharia metabólica
Facilitador de sacarose
Fosforilase de sacarose
Abstract in Portuguese
A indústria biotecnológica vem ganhando destaque em função das negativas atreladas ao uso de recursos fósseis. Nesse cenário, o Brasil se destaca por seu programa de produção de bioetanol bem estabelecido e pelo uso de cana-de-açúcar como matéria prima barata. O presente trabalho construiu Saccharomyces cerevisiae transgênicas para aprodução de compostos de interesse econômico cuja biossíntese consome energia livre (ATP). Para tanto, a expressão de proteínas heterólogas e engenharia evolutiva foram realizadas em levedura de modo que a produção de determinados compostos se torne energicamente viável.
Title in English
Metabolic engineering of Saccharomyces cerevisiae aimed at improving the energetic yield of sucrose metabolism.
Keywords in English
Saccharomyces cerevisiae
Bioenergetics
Evolutionary engineering
Metabolic engineering
Sucrose Facilitator
Sucrose phosphorylase
Abstract in English
The biotechs industry is a growing field since fossil resources are being attached to ecological and geopolitical constraints. In this scenario, Brazil has a major role due to its large experience in the bioethanol industry and sugarcane use as a cheap feedstock. The aim of this work is to optimize Saccharomyces cerevisiae allowing them to occupy a new niche: the production of economically valuable chemicals that require cellular free energy (ATP) on their biosynthesis. In this context, heterologous protein expression and evolutionary engineering were done. Therefore, this work will potentially contribute to make certain energy demanding chemicals production economically viable.
 
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Publishing Date
2018-10-22
 
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