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Master's Dissertation
DOI
https://doi.org/10.11606/D.85.2011.tde-04082011-085145
Document
Author
Full name
Marcelo Miyada Redigolo
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Sato, Ivone Mulako (President)
Tabacniks, Manfredo Harri
Zamboni, Cibele Bugno
Title in Portuguese
Determinação de elementos químicos inorgânicos em amostras de sangue total humano e de animais de experimentação (hamster dourado e cavalo da raça crioula) pela técnica de fluorescência de raios X(EDXRF)
Keywords in Portuguese
determinação de elementos inorgânicos
fluorescência de raios X
intervalos de referência
sangue total animal
sangue total humano
Abstract in Portuguese
O sangue é uma suspensão de células contidas num líquido complexo chamado plasma. O termo sangue total refere-se a amostras de sangue com a totalidade de seus constituintes, parte sólida e líquida. Sendo os elementos químicos responsáveis por funções essenciais, como regulação osmótica, frequência cardíaca e contratibilidade, coagulação sanguínea e excitabilidade neuromuscular. A determinação de elementos químicos em fluidos corporais como sangue, soro, plasma, tecido e urina é usada como monitor de parte ou de todo o organismo. Nesse trabalho, utilizou-se a técnica de fluorescência de raios X (EDXRF) para análise de amostras de sangue total humano e animal, hamsters da espécie dourada (Mesocricetus auratus) e cavalos da raça crioula (Equus caballus). Nas amostras de sangue humano, foram determinados intervalos de referência de Na (1788 - 1826 μg g-1), Mg (63 - 75 μg g-1), P (602 - 676 μg g-1), S (1519 - 1718 μg g-1), Cl (2743 - 2867 μg g-1), K (1508 - 1630 μg g-1), Ca (214 - 228 μg g-1), Cu (4 - 6 μg g-1) e Zn (1 - 3 μg g-1). Foram determinados intervalos de referência de Na (1714 - 1819 μg g-1), Mg (51 - 79 μg g-1), P (970 - 1080 μg g-1), S (1231 - 1739 μg g-1), Cl (2775 - 2865 μg g-1), K (1968 - 2248 μg g-1), Ca (209 - 257 μg g-1), Cu (4 - 6 μg g-1) e Zn (3 - 5 μg g-1) para amostras de sangue de hamster dourado. As amostras de sangue de cavalo da raça crioula apresentaram os intervalos de: Na (1955 - 2013 μg g-1), Mg (51 - 75 μg g-1), P (443 - 476 μg g-1), S (1038 - 1140 μg g-1), Cl (2388 - 2574 μg g-1), K (1678 - 1753 μg g-1), Ca (202 - 213 μg g-1), Cu (4,1 - 4,5 μg g-1) e Zn (2,0 - 2,2 μg g-1). Estudo comparativo dos resultados entre as técnicas de análise por ativação neutrônica (NAA) e EDXRF indica a igualdade de desempenho das técnicas analíticas na análise de matrizes biológicas. Os resultados contribuem no estabelecimento de intervalos de referência para a população brasileira saudável e para as referidas espécies de animais.
Title in English
Inorganic elements determination in human and animal whole blood samples by X-ray fluorescence technique (EDXRF)
Keywords in English
animal whole blood
human whole blood
inorganic elements determination
reference intervals
X-ray fluorescence
Abstract in English
Blood is a suspension of cells contained in a complex liquid called plasma. The term whole blood refers to samples with both solid and liquid parts. Inorganic elements are responsible for essential functions, such as osmotic regulation, cardiac frequency and contractibility, blood clotting and neuromuscular excitability. The determination of inorganic elements in corporeal fluids such as blood, serum, plasma, tissue and urine is used as a monitor for a part or the whole organism. In this work, the X-Ray fluorescence technique (EDXRF) was used for the determination of inorganic elements in whole blood samples from humans and animals (golden hamsters, Mesocricetus auratus and crioula breed horses, Equus caballus). The reference intervals of Na (1788 - 1826 μg g-1), Mg (63 - 75 μg g-1), P (602 - 676 μg g-1), S (1519 - 1718 μg g-1), Cl (2743 - 2867 μg g-1), K (1508 - 1630 μg g-1), Ca (214 - 228 μg g-1), Cu (4 -6 μg g-1) e Zn (1 - 3 μg g-1) were determined for human blood. The reference intervals, for golden hamster blood were found to be: Na (1714 - 1819 μg g-1), Mg (51 - 79 μg g-1), P (970 - 1080 μg g-1), S (1231 - 1739 μg g-1), Cl (2775 - 2865 μg g-1), K (1968 - 2248 μg g-1), Ca (209 - 257 μg g-1), Cu (4 - 6 μg g-1) e Zn (3 -5 μg g-1). The reference intervals, for crioula breed horse blood, showed to be: Na (1955 - 2013 μg g-1), Mg (51 - 75 μg g-1), P (443 - 476 μg g-1), S (1038 - 1140 μg g-1), Cl (2388 - 2574 μg g-1), K (1678 - 1753 μg g-1), Ca (202 - 213 μg g-1), Cu (4,1 - 4,5 μg g-1) e Zn (2,0 - 2,2 μg g-1). Comparative study between NAA and EDXRF, both techniques showed the same performance for the analyses of biological matrices. The results contribute for the establishment of reference intervals for the Brazilian healthy population and the referred animal species.
 
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Publishing Date
2011-08-08
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • BAPTISTA, Tatyana S., et al. Comparative study of inorganic elements determination in whole blood from Crioula breed horse by EDX : RF and and NAA analytical techniques. Journal of Radioanalytical and Nuclear Chemistry [online], 2011, vol. 274, n. 1. Tatyana S. Baptista and Marcelo M. Redígolo contributed equally to this work.. [cited 2011-11-01]. Available from : <http://www.springerlink.com/content/80863718v4745u01/>
All rights of the thesis/dissertation are from the authors
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