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Master's Dissertation
DOI
https://doi.org/10.11606/D.85.2010.tde-29082011-104826
Document
Author
Full name
Silvio Carneiro da Cunha Filho
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Nascimento, Nanci do (President)
Cursino, Sylvia Regina Temer
Higa, Olga Zazuco
Title in Portuguese
Epidemiologia e estudo dos fatores responsáveis pela espongiose ocular no município de Araguatins -TO
Keywords in Portuguese
Araguatins
cegueira
citotoxicidade
cromatografia
doença ocular de etiologia desconhecida
esponjas de água-doce
poríferas
Abstract in Portuguese
Em outubro de 2005 a notificação de 17 casos de doença ocular de etiologia desconhecida, envolvendo, em sua maioria, a população infantil da cidade de Araguatins/TO, levou as autoridades locais a pedirem ajuda a Secretaria de Estado da Saúde do Estado do Tocantins no intuito de descobrirem sua etiologia, tratamento e prevenção. Nos pacientes acometidos, os sinais freqüentemente observados foram: intensa hiperemia conjutival, granuloma, episclerite, infiltrado corneano periférico. Na anamnese realizada nos pacientes foi observado que todos tiveram contato com as águas do Rio Araguaia. Os resultados obtidos a partir do processamento das amostras de água, sedimentos e substratos particularmente na vegetação marginal inundada, permitiram confirmar a hipótese de que deveria haver uma fauna rica de esponjas no Araguaia, no trecho fronteiro à cidade de Araguatins A presença intraocular de espícula de esponja de água doce das espécies Drulia uruguayensis e Oncosclera navicela foi confirmada em material avaliado histopatologicamente proveniente de três pacientes que haviam sido submetidos a lensectomia, sugerindo que espículas de água doce poderiam ser um surpreendente novo agente etiológico de patologia ocular. Assim, neste estudo foram realizados ensaios de citotoxicidade com amostras de duas espécies de esponjas coletadas no local. Os resultados indicam que os extratos das esponjas após filtração em filtro Millipore 0,45 μm continuaram apresentando atividade citotóxica, sugerindo haver um componente SOLÚVEL, e não somente espículas, capaz de induzir morte celular na população de células utilizadas. Os indivíduos acometidos foram na maioria do sexo masculino com idade entre 05 e 14 anos.
Title in English
Epidemiology and study of the factors responsible for spongiosis ocular in the city of Araguatins TO
Keywords in English
Araguatins
chromatography
eyes disease of unknown etiology
Abstract in English
In October 2005 the notification of 17 cases of eyes disease of unknown etiology, involving, in its majority, the infant population of the city of Araguatins/TO, led the local authorities to ask help to the Secretariate of State of Health of the State of Tocantins in order to discover their etiology, treatment and prevention. In affected patients, the signs frequently observed were: intense hyperemia conjutival, granuloma, episclerite, peripheral cornea infiltrated. In the anamnesis performed in patients was observed that all of them had contact with the waters of the Araguaia river. The results obtained from processing samples of water, sediments and substrates particularly in the vegetation marginal flooded, allowed confirming the hypothesis that there should be a rich fauna sponges in Araguaia, close to the city of Araguatins The presence of spicula of intraocular sponge species of freshwater Drulia uruguayensis and Oncosclera navicela was confirmed in material evaluated histopathologically from three patients who had undergone lensectomy, suggesting that "spikes freshwater could be a surprising new etiologic agent of pathology ocular". Thus, in this study was performed cytotoxicity assays with samples from two species of sponges collected in place. The results indicate that the extracts of sponges after filtration Millipore 0.45 μm continued presenting cytotoxic activity, suggesting there is a SOLUBLE component, and not only spikes, capable of inducing cellular death in the population of cells used. The individuals affected were the majority of males aged between 05 and 14 years.
 
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Publishing Date
2011-08-31
 
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