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Mémoire de Maîtrise
DOI
https://doi.org/10.11606/D.82.2005.tde-28062005-104659
Document
Auteur
Nom complet
Andrezza Furquim da Cruz
Adresse Mail
Unité de l'USP
Domain de Connaissance
Date de Soutenance
Editeur
São Carlos, 2005
Directeur
Jury
Ferreira, Affonso Luiz (Président)
Rosa, Stela Marcia Mattiello Gonçalves
Silva, Orivaldo Lopes da
Titre en portugais
Estudo dos efeitos da irradiação pulsada de baixa intensidade sobre o desenvolvimento de segmentos de coluna vertebral implantados em camundongos isogênicos
Mots-clés en portugais
ossificação endocondral
ultra-som pulsado de baixa intensidade
Resumé en portugais
Estudo dos efeitos da irradiação ultra-sônica pulsada de baixa intensidade sobre o desenvolvimento de segmentos da coluna vertebral implantados em camundongos isogênicos
Titre en anglais
Low-intensity pulsed ultrasonic irradiation effects on development of vertebral column segments implanted in isogenics mice
Mots-clés en anglais
endochondral ossification
low-intensity pulsed ultrasound
Resumé en anglais
The aim of the present research was evaluate the action of low-intensity pulsed ultrasonic irradiation on development and ossification of vertebral column blastemas, through mice new born tail segments implants in adult receptor isogenic mice lineages C57BL/6 and Balb/C. New born tail segments were implanted in subcutaneous tissues of adults isogenics mice C57BL/6 and intramuscularly in adults isogenics mice Balb/C. After 24 hours the implant, the animals in both groups, were stimulated with the low-intensity pulsed ultrasound (LIPUS), 10 minutes per day. After 5 days of stimulus, the receptors animals were death and had the implanted tails removed for histopathology, trhrough Periodic Acid Schiff plus Alcian Blue (PAS/AB), Masson's tricomico and hem lumen-eosin (HE). The group of animals stimulated which received subcutaneous implant presented the implant with accelerated in inter-vertebral diks, faces to chondrocytes organization and chondroblastes findings in cartilaginous matrix, showing maturity in fiber cartilages arrangements. It was observed too, a collagen fibers enlargement in the disks fibro cartilage which show more dense next the pulposo nucleus in the segments implanted and stimulated, compared with the controls segments. The areas of the segments were available by Fisher's test and Student's t. It didn't watch significant differences in the vertebral bodies' areas sizes in the animals which implant was subcutaneous (p'< OU = '0.05). In the intramuscular implants animals group, it was observed a major velocity in the hialuronic acid matrix formation in the intervertebral disks after LIPUS stimulus. Moreover, the fibrous ring fiber cartilages' were better organized with large number of cells inside the cartilaginous matrix compared with the controls segments. With regard to the vertebral bodies in the intramuscular implants, it's not verifies significant increases in the areas (p'< OU =' 0,05). The findings allow conclude that LIPUS should promote the ontogenetic differentation of the structural components of the mice vertebral column more quickly, trhough vertebras endochondral ossification microscopic evaluation and the inter-vertebral disks components differentiation, follow the methodology employed.
 
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andrezza_furquim.pdf (9.20 Mbytes)
Date de Publication
2006-10-16
 
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