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Master's Dissertation
DOI
10.11606/D.75.2006.tde-11042007-110413
Document
Author
Full name
Fabiana Cristina Vila
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2006
Supervisor
Committee
Yariwake, Janete Harumi (President)
Silva, Maria Fatima das Graças Fernandes da
Thiemann, Otavio Henrique
Title in Portuguese
Identificação dos flavonóides com atividade antioxidante da cana-de-açúcar (Saccharum officinarum L.)
Keywords in Portuguese
antioxidante
CLAE
flavonóides
Abstract in Portuguese
No presente trabalho estudou-se por métodos analíticos (CCD e CLAE/UV) a atividade antioxidante dos flavonóides presentes na cana-de-açúcar (Saccharum officinarum L.), visando sua possível utilização como alimento funcional. As técnicas CLAE/UV e microfracionamento por CLAE permitiram o fracionamento e o isolamento dos flavonóides, os quais foram analisados por CCD utilizando reagentes específicos para detecção de substâncias antioxidantes. Os resultados obtidos permitiram identificar os flavonóides da cana-de-açúcar (folhas e garapa) com atividade antioxidante, através da comparação dos espectros de UV/DAD e tempo de retenção: orientina-O-ramnosídeo nas folhas e schaftosídeo, isoschaftosídeo, diosmina-8-C-glicosídeo, orientina e 4',5'-di-O-metil-luteolina-8-C-glicosídeo na garapa. Estes resultados justificam estudos nutricionais e/ou farmacológicos mais aprofundados a fim de classificar (ou não) a cana-de-açúcar como alimento funcional.
Title in English
Identification of the flavonoids with antioxidants activity of the sugar cane (SAccharum officinarum L.)
Keywords in English
antioxidants
flavonoids
HPLC
Abstract in English
In the present study, analytical methods (TLC and HPLC/UV) were used to evaluate the antioxidant activity of the flavonoids of sugar cane (Saccharum officinarum L.) regarding to its possible use as a functional food. HPLC/UV and HPLC microfractionation techniques allowed fractionation and isolation of the flavonoids, which were evaluated by TLC using specific reagents to detect the antioxidant compounds. The results allowed to identify the flavonoids of sugar cane with antioxidant activity, based on comparison of UV-DAD spectra and retention time: orientin-O-rhamnoside in the leaves and schaftoside, isoschaftoside, diosmetin-8-C-glycoside, orientin and 4'-5'-dimethyl-luteolin-8-C-glycoside in the juice. Theses results justify more detailed nutricional and pharmacologic studies to classify (or not) sugar cane as a functional food.
 
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FabianaVila.pdf (982.70 Kbytes)
Publishing Date
2007-04-16
 
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