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Master's Dissertation
DOI
10.11606/D.74.2017.tde-16032017-103812
Document
Author
Full name
Paula Irusta Ferreira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Pirassununga, 2016
Supervisor
Committee
Leite Dellova, Deise Carla Almeida (President)
Ferreira, Helena Lage
Guerra, José Luiz
Title in Portuguese
Estudo de respostas fibróticas e apoptóticas em rins de ratos tratados com aldosterona
Keywords in Portuguese
Aldo
BAX
Injúria renal
PAI-1
TGF-β1
Abstract in Portuguese
Procurando compreender o envolvimento da aldosterona (Aldo) na injúria renal, o objetivo deste projeto foi avaliar o efeito do tratamento crônico com Aldo sobre a função renal e a histologia das arteríolas renais, procurando correlacionar os achados com a expressão de genes reguladores do processo de fibrose e apoptose. A Aldo não alterou os parâmetros fisiológicos, PA, ritmo de filtração glomerular (estimado pela depuração plasmática de creatinina), proteinúria e a morfologia das arteríolas corticais. No entanto, aumentou a expressão do RNAm para TGF-β1, PAI-1 e BAX no tecido renal, além da contagem de células TUNEL positivas nos glomérulos. O antagonismo ao receptor MR (pelo uso da espironolactona) aboliu o efeito hormonal somente sobre a expressão do RNAm para BAX e a marcação do DNA degradado (TUNEL), enquanto que o antagonismo ao GR (pelo uso do RU 486) reduziu ou aboliu todos os efeitos da Aldo. Os resultados indicam que a Aldo pode induzir respostas precoces sobre o remodelamento do tecido renal, sem ainda comprometer a função renal ou alterar a PA. Essas respostas foram independentes da sobrecarga de sal e ocorreram por um mecanismo que envolveu os receptores MR e GR.
Title in English
Study of fibrotic and apoptotic responses in rat kidney after aldosterone treatment
Keywords in English
Aldo
BAX
PAI-1
Renal injury
TGF-β1
Abstract in English
In order to understand the aldosterone (Aldo) involvement in renal injury, the objective of this project was to evaluate the effect of Aldo chronic treatment on renal function and renal arterioles histology, trying to correlate the findings with the regulatory genes of fibrosis and apoptosis. Aldo did not change the physiological parameters, BP, glomerular filtration rate (estimated by creatinina clearance), proteinuria and cortical arterioles morphology. However, Aldo increased mRNA expression for TGF-β1, PAI-1 and BAX in renal tissue, as well as TUNEL-positive cell count in glomeruli. MR receptor antagonism (by spironolactone) abolished only the hormonal effect on the mRNA expression for BAX and degraded DNA labeling (TUNEL); whereas GR antagonism (by RU 486) reduced or abolished all Aldo effects. The results indicated that Aldo can induce early responses on renal tissue remodeling, without altering renal function or BP. These responses were salt independent and involved MR and GR receptors.
 
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ME8842501COR.pdf (1.15 Mbytes)
Publishing Date
2017-03-22
 
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