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Master's Dissertation
DOI
https://doi.org/10.11606/D.74.2003.tde-23042004-135041
Document
Author
Full name
Nilton Pedro dos Santos
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
Pirassununga, 2003
Supervisor
Committee
Blazquez, Francisco Javier Hernandez (President)
Meirelles, Flavio Vieira
Silva, Jose Roberto Machado Cunha da
Title in Portuguese
Regeneração hepática em bagre africano (Clarias gariepinus) após hepatectomia parcial.
Keywords in Portuguese
BrdU
ductos biliares
fígado
hepatectomia
regeneração hepática
teleósteos
Abstract in Portuguese
O fígado é um órgão importante para se analisar como o animal reage a agressões químicas. Um dos métodos utlizado para estudar a regeneração hepática é a hepatectomia parcial (HP). Assim, nosso objetivo foi verificar a influência da hepatectomia de 30% e de 70% sobre a taxa de proliferação das células hepática medida pela incorporação de BrdU na região da incisão e no resto do fígado e a proliferação de ductos biliares e de células epiteliais pré-ductulares biliares (CEPDBs) com o uso de uma combinação de 2 anticorpos específicos para citoqueratinas humanas, AE1/AE3. O pico de proliferação nos animais que sofreram HP de 30% do parênquima hepático deu-se após 1 dia da cirurgia enquanto que os animais que sofreram a HP de 70% o pico ocorreu no terceiro dia após a cirurgia.O índice de proliferação foi semelhante para a região próxima ou distante do corte. Para ambas as HP não houve diferença na regeneração do tecido retirado próximo ou distante da área de incisão. Nas regiões distantes da HP havia maior número de ductos havendo o pico do número de ductos coincidindo com o pico de proliferação celular de ambas as HP. Depois da HP o número de CEPDBs cresce consideravelmente. Os pico de proliferação também ocorreram no primeiro e terceiro dia para a HP de 30% e de 70% respectivamente, sendo mais altos na região distante do corte. Assim, durante a regeneração do fígado de C. gariepinus ocorre hiperplasia compensatória por proliferação de hepatócitos, de ductos biliares e de CEPDBs. A hepatectomia de 30% gerou uma resposta regenerativa intensa e é menos traumática para o animal, a região distante do corte reage mais intensamente que a região próxima do corte no que se refere aos ductos biliares e as CEPDBs.
Title in English
Hepatic regeneration in african catfish (Clarias gariepinus) after partial hepatectomy.
Keywords in English
biliary ducts
BrDU
hepatectomy
hepatic regeneration
liver
teleost
Abstract in English
The liver is an important organ in studies aimed to the verification of animals reactions to chemical injuries. The partial hepatectomy (PH) is one of the methods that are usually employed in hepatic regeneration experiments. Our objectives in this work were to verify the influence of 30% and 70% PH on the hepatic cells proliferation index as it is measured by BrdU nuclear uptake in two regions: close to the hepatic surgery region and in a farther region. The biliary ducts and bile preductular epithelial cells (BPDECs) proliferation was quantified with a combination of two antibodies against the human cytokeratins AE1/AE3. The proliferation index peak in 30% hepatectomy animals occurred one day after the surgery, but in 70% hepatectomy animals the peak was only observed after three days from the surgery. Differences between the proliferation index of the close and the far region were not observed and likewise histological differences were absent. In the regions far from the PH site more biliary ducts were counted than in closer regions, but the peaks were coincident in both regions with the hepatocytes proliferation index. It was also observed that the quantity of BPDECs increased. The peaks occurred in the first and third days for the 30% and 70% PH respectively, and both peaks were higher in the region far from the PH site. We may conclude that C. gariepinus liver regeneration after 30% and 70% PH occurs by means of compensatory hyperplasia by hepatocytes, biliary ducts and BPDECs proliferation. The 30% HP stimulated a strong regenerative response and is less traumatic to the animals. The regions far from the PH site reacts more intensely that the close region concerning the stimulation of biliary ducts and BPDECs proliferation.
 
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Publishing Date
2004-04-23
 
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