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Master's Dissertation
DOI
https://doi.org/10.11606/D.55.2009.tde-18062009-084415
Document
Author
Full name
Cristina Teixeira de Oliveira
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Carlos, 2009
Supervisor
Committee
Telles, Guilherme Pimentel (President)
Delbem, Alexandre Cláudio Botazzo
Silva, Felipe Rodrigues da
Title in Portuguese
Método para melhoria da eficiência na identificação computacional de RNAs não-codificantes
Keywords in Portuguese
Bioinformática
Infernal
RNAs não-codificantes
Viena
Abstract in Portuguese
Até pouco tempo acreditava-se que a maioria das moléculas de RNA estava relacionada à tradução de proteínas. Porém, descobriu-se que outros tipos de moléculas de RNA que não são traduzidas estão presentes em muitos organismos diferentes e afetam uma variedade de processos moleculares, são os chamados RNAs não-codificantes (ncRNAs). Apesar de sua importância funcional, os métodos biológicos e computacionais para a detecção e caracterização de RNAs não-codificantes ainda são imprecisos e incompletos. A identificação de novas espécies de ncRNAs é difícil através de procedimentos experimentais e as técnicas computacionais existentes são lentas. O objetivo deste trabalho foi obter uma ferramenta mais eficiente para a comparação de uma seqüência de RNA não-codificante contra um banco de seqüências. Para isso foi proposto e implementado um modelo para identificação computacional de ncRNAs com apoio dos pacote Viena e Infernal e foram realizados experimentos para avaliá-lo
Title in English
Method to obtain a more efficient tool that compares a non-coding RNA sequence against a sequence database
Keywords in English
Bioinformatics
Infernal
Noncoding RNAs
Viena
Abstract in English
Until recently it was generally accepted that most RNA molecules were involved in the translation process. However, it was discovered that many types of untranslated RNA molecules are present in many different organisms and they are related to a wide variety of molecular processes. These molecules are called non-coding RNAs (ncRNAs). Despite their functional importance, the biological and computational methods to detect and identify non-coding RNAs are still imprecise and incomplete. The discovery of new ncRNAs species is difficult through experimental procedures and the existing computational techniques are slow. This project aimed at obtaining a more efficient tool that compares a non-coding RNA sequence against a sequence database. In order to achieve this, a computational model for ncRNAs identification using the Vienna and Infernal packages has been proposed and implemented. Experiments were conduced to evaluate the model
 
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Publishing Date
2009-06-18
 
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