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Master's Dissertation
DOI
https://doi.org/10.11606/D.5.2007.tde-12022008-130243
Document
Author
Full name
Adriana Freire Machado
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Machado, Clarisse Martins (President)
Costa, Silvia Figueiredo
Raboni, Sonia Mara
Title in Portuguese
Caracterização molecular das cepas do Vírus Sincicial Respiratório identificadas nos anos de 2001 e 2002 em unidade de transplante de células tronco hematopoéticas
Keywords in Portuguese
Células-tronco hematopoéticas
Epidemiologia molecular
Hospedeiro imunocomprometido
Infecção hospitalar
Infecções por respiratório sincicial/transmissão
Transplante de medula óssea
Abstract in Portuguese
O Vírus Sincicial Respiratório (RSV) é reconhecido como agente causador de infecção nosocomial entre receptores de pacientes de células-tronco hematopoéticas causando morbidade e mortalidade consideráveis nesses pacientes. O objetivo desse estudo foi caracterizar as cepas do RSV isoladas de receptores de transplante de células-tronco hematopoéticas (TCTH) do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo durante sua estação. As cepas do RSV foram tipadas (em grupo A ou B) e genotipadas. Das sete cepas analisadas dos receptores de TCTH durante o ano de 2001, somente duas pertenciam ao grupo B, as outras cinco eram pertencentes ao grupo A. Dessas sete cepas, três eram altamente relacionadas e haviam infectados pacientes que freqüentavam o ambulatório. Em 2002, das doze cepas analisadas, três pertenciam ao grupo A e as outras nove pertenciam ao grupo B. Sete cepas eram altamente relacionadas entre elas e eram também de pacientes de ambulatório sugerindo que a transmissão em hospital-dia era mais provável. Enfim, múltiplços genótipos do RSV co-circularam nas unidades de TCTH (ambulatório e enfermaria) do Hospital das Clínicas entre 2001 e 2002. A transmissão nosocomial foi mais provável ocorrer no ambulatório da unidade de TCTH quando comparada à enfermaria. Políticas de controle de infecção devem ser também implementadas em ambulatórios para evitar transmissão nosocomial do RSV e outros vírus respiratórios em pacientes de ambulatório.
Title in English
Molecular characterization of strains of Respiratory Syncytial Virus in Hematopoietic Stem Cell Transplant Unit identified in 2001 and 2002
Keywords in English
BBone marrow transplantation
Cross infection
Hematopoietic stem cells
Immunocompromissed host
Molecular epidemiology
Respiratory syncytial virus/transmission
Abstract in English
Respiratory Syncytial Virus is recognized as the leading cause of nosocomial respiratory infection among recipients of hematopoietic stem cell transplant (HSCT) causing considerable morbity and mortality among theses patients. The aim this study was characterize the strains of Respiratory Syncytial Virus in recipients Hematopoietic Stem Cell Transplant Unit (HSCT) at Hospital das Clínicas, University of São Paulo Medical School during RSV season in symptomatic HSCT recipients at Hospital das Clínicas. The strains of RSV was typed (in group A or B) and genotyped. Of the seven strains analyzed from HSCT recipients during 2001, only two belonged to group B, the other five belonged to group A. Of these seven strains, three were closely related and were from outpatients. In 2002 , of the twelve strains analyzed, three belonged to group A and the other nine belonged to group B. Seven strains were closely related and were also from outpatients suggesting that nosocomial transmission in hospital-day was more likely. In conclusion, multiples genotypes of RSV co-circulated in the Hematopoietic Stem Cell Transplant units (ward and dayhospital) of Hospital das Clínicas between 2001 and 2002. Nosocomial transmission was more likely to occur at the HSCT Day-hospital as compared to the HSCT ward. Infection control practices should be also implemented at Day-hospital Units to avoid nosocomial transmission of RSV and other respiratory viruses in outpatient units.
 
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AdrianaFMachado.pdf (1.66 Mbytes)
Publishing Date
2008-03-14
 
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