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Doctoral Thesis
DOI
https://doi.org/10.11606/T.46.2000.tde-18092015-163705
Document
Author
Full name
Euclides Matheucci Junior
E-mail
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2000
Supervisor
Committee
Dorry, Hamza Fahmi Ali El (President)
Farah, Shaker Chuck
Marques, Marilis do Valle
Silva, Aline Maria da
Terenzi, Hernan Francisco
Title in Portuguese
Isolamento e análise funcional do gene que codifica uma proteína serina-treonina quinase que modula a expressão de genes regulados por carboidratos em Trichoderma reesei
Keywords in Portuguese
Biologia molecular
Carboidratos (Metabolismo)
Clonagem
DNA
Fungo filamentoso
Metabolismo
Proteínas (Metabolismo)
Quinase
Snf-1
Abstract in Portuguese
O gene TrSNF1, homólogo aos membros da subfamília das proteínas serina-treonina quinases ativadas por AMP (AMPK) e relacionadas a SNF1, foi isolado do fungo filamentoso trichoderma reesei. A seqüência de aminoácidos putativa possui um domínio de quinase com 42% de identidade e 59% de similaridade com outras proteínas quinases da mesma subfamília. Em S. cerevisiae a SNFl é essencial para a expressão de genes reprimidos por glicose, em resposta a privação de glicose do meio de cultura. A expressão de TrSNFl em levedura mutante para SNF1, restaura a função de SNF1. A expressão de um antisense de TrSNFl em T. reesei causa um atraso na expressão do gene regulado por glicose, CBHI. Além disso, em experimento utilizando matrizes de DNA foi possível observar uma alteração da tendência global da expressão gênica entre a cepa selvagem e a cepa antisense. A observação da homologia estrutural com proteínas quinase da mesma subfamília, a similaridade funcional com SNFl de S. cerevisiae, e a alteração no padrão da expressão gênica in vivo, sugerem que TrSNFl pode estar envolvido na regulação do metabolismo de carboidratos em T. reesei.
Title in English
Isolation and functional analysis of the gene encoding a serine-threonine protein kinase that modulates the expression of genes regulated by carbohydrates Trichoderma reesei
Keywords in English
Carbohydrates (Metabolism)
Cloning
DNA
Filamentous fungus
Kinase
Metabolism
Molecular biology
Proteins (Metabolism)
Snf-1
Abstract in English
A gene homologue to the members of the AMP-activated/SNFl protein kinase subfamily, TrSNF1, was isolated from the filamentous fungus Trichoderma reesei. The predicted protein of 692 amino acids has a kinase domain, that share 42 % identity and 59 % similarity to that of serine/threonine protein kinase of this family. In Saccharomyces cerevisiae, the SNFl protein kinase is required for expression of glucose repressed genes in response to withdrawal of glucose from the medium. Expression of the Trichoderma reesei SNF1-related sequence in yeast SNFl mutant restores SNFl function. The TrSNFl antisense expression in T. reesei causes a control alteration in the glucose-regulated gene CBHI. The observed structural identity with the AMP-activated/SNFl protein kinase subfamily, and the functional similarity to the yeast SNFl suggest that the TrSNFl may be involved in the regulation of sugar metabolism in Trichoderma reesei.
 
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Publishing Date
2015-09-18
 
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