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Master's Dissertation
DOI
https://doi.org/10.11606/D.46.2018.tde-01102018-164651
Document
Author
Full name
Leandro Marcio Moreira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2002
Supervisor
Committee
Silva, Ana Claudia Rasera da (President)
Menck, Carlos Frederico Martins
Souza, Glaucia Mendes
Title in Portuguese
Análise comparativa entre os genomas dos fitopatógenos Xylella fastidiosa e Xanthomonas axonopodis pv. citri
Keywords in Portuguese
Análise comparativa
Bactérias aeróbicas gram-negativas (Análise genética)
Fitopatógenos
Genomas (Análise)
Metabolismo
Xanthomonas
Xanthomonas (Análise genética)
Xylella
Abstract in Portuguese
Xylella fastidiosa (Xf) e Xanthomonas axonopodis pv. citri (Xac) são gama proteobactérias gram negativas, responsáveis por grandes perdas econômicas no setor citrícola brasileiro. Com seus genomas seqüenciados e anotados, fizemos uma análise comparativa entre suas composições gênicas e seus ambientes de vida. Xac apresenta um genoma de 5.2Mb contra 2.7Mb de Xf Isto reflete no número de genes (4432 contra 2838) que acabam refletindo em uma maior complexidade metabólica de Xac, caracterizada por: uma extensa gama de genes de degradação de parede celular (44), biossíntese de proteases (92), genes de funções regulatórias (296), um completo metabolismo energético (209), quimiotático (inexistente em Xf) e secretório (presença dos tipos I, II, III e IV , sendo o II em duplicata), além de um grande número de genes envolvidos com captação de ferro (65), fazem de Xac um patógeno de alto poder invasivo e de rápida propagação e virulência Em contrapartida, Xf por não possuir a complexidade supracitada, parece ter seus recursos adaptados ao ambiente em que vive, como por exemplo um alto número de genes envolvidos com biossíntese de pili, que associado à biossíntese de goma, favorecem sua adesão nas glândulas salivares do vetor (cigarrinha) e a formação de aglomerados celulares responsáveis pelo entupimento dos vasos que levam às patologias decorrentes do evento.
Title in English
Comparative analysis between the genomes of the phytopathogens Xylella fastidiosa and Xanthomonas axonopodis pv. citri
Keywords in English
Comparative analysis
Genomes (Analysis)
Gram-negative aerobic bacteria (Genetic analysis)
Metabolism
Phytopathogens
Xanthomonas
Xanthomonas (Genetic analysis)
Xylella
Abstract in English
Xylella fastidiosa (Xf) and Xanthomonas axonopodis pv. citri Xac are gram negative gamma proteobacteria, responsible for great economical losses in the Brazilian citrus sector. With their sequenced and annotated genomes, we have done a comparative analysis between their genetic composition and life habitat. Xac displays a genome of 5.2Mb against 2. 7Mb of Xf. This reflects the number of genes (4432 against 2838) which results in a greater metabolic complexity of Xac, characterized by: a wide range of genes of cell wall degradation (44), biosynthesis of proteases (92), many genes of regulatory functions (296), a complex energy metabolism (209), chemotatic (absent in Xf) and secretory systerns (presence of types I, II, III and IV, type II in duplicate), besides a great number of genes involved in iron acquisition (65), make of Xac a pathogen of high invasive power and of quick spreading and virulence. In the other hand Xf, due to the lack of the complexity just cited, seems to have its resources adapted to the habitat in which it lives, as for example a large number of genes involved in pili biosynthesis, that associated with gum biosynthesis, favor its adhesion to the salivary glands of the vector (sharpshooter) and the formation of cellular agglomerations responsible for the blockage of the vessels which leads to the pathologies resulted from this event.
 
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Publishing Date
2018-10-01
 
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