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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2009.tde-22012010-100738
Document
Author
Full name
Nicolau Agostinho Sambé
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Metzger, Martin Andreas (President)
Camarini, Rosana
Pires, Raquel Simoni
Toledo, Claudio Antonio Barbosa de
Torrão, Andréa da Silva
Title in Portuguese
Distribuição de receptores ionotrópicos de glutamato e sua co-localização com a fosfoproteína neural DARPP-32 no córtex pré-frontal de ratos.
Keywords in Portuguese
AMPA
Córtex pré-frontal
DARPP-32
Dopamina
Fisiologia
Fosfoproteínas
Glutamatos
Neurotransmissores
Ratos
Abstract in Portuguese
O córtex pré-frontal medial (PFCm) é caracterizado por entradas glutamatérgicas e dopaminérgicas que convergem sobre os mesmos neurônios alvos. Devido à escassa informação sobre as bases anatômicas das interações entre a dopamina (DA) e o glutamato (Glu), mapeamos a distribuição de subunidades (Su) de receptores (Rs) de Glu do tipo AMPA, NMDA e kainato no PFCm e investigamos a sua expressão em neurônios contendo a fosfoproteína DARPP-32 e em interneurônios. Os resultados mostram que as Su GluR2/3 dos Rs do tipo AMPA são as mais amplamente distribuídas no PFCm e expressas em todos os neurônios DARPP-32+. GluR2/3 é também amplamente co-localizado com as Su NMDAR1 dos Rs de Glu do tipo NMDA e GluR5/6/7 dos Rs do tipo kainato. Em contraste, as Su GluR1 e GluR4 são somente fracamente expressos no PFCm e não são co-localizados com DARPP-32, porém com GABA ou parvalbumina. Os resultados indicam que as Su GluR2/3, NMDAR1 e GluR5/6/7 são amplamente expressos em neurônios piramidais DARPP-32+ enquanto GluR1 e GluR4 são predominantemente expressos em interneurônios do PFCm.
Title in English
Distribution of ionotropic glutamate receptors and their co-localization with the phosphoprotein DARPP-32 in the medial prefrontal córtex of rats.
Keywords in English
AMPA
DARPP-32
Dopamine
Glutamate
Neurotransmitters
Phosphoproteins
Physiology
Pre frontal cortex
Rats
Abstract in English
The medial prefrontal cortex (PFCm) is characterized by glutamatergic and dopaminergic afferents that converge on the same target neurons. Since there is only limited information about the anatomical bases for interactions between dopamine (DA) and glutamate (Glu), we mapped the distribution of AMPA, NMDA and kainate Glu receptor (Rs) subunits (Su) in the PFcm and investigated their expression in neurons containing the phosphprotein DARPP-32 and in interneurons. Results show that the Su GluR2/3 of AMPA type Rs are the most prominently distributed in the PFCm and expressed in all neurons DARPP-32+. GluR2/3 is also widely co-localized with the NMDA type Su NMDAR1 and the Kainate Su GluR5/6/7. In contrast, the Su GluR1 and GluR4 are only weakly expressed in the PFCm and are not colocalized with DARPP-32 but with GABA or parvalbumin. Results indicate that the Su GluR2/3, NMDAR1, and GluR5/6/7 are prominently expressed in DARPP-32+ pyramidal neurons, whereas GluR1 and GluR4 are predominantly expressed by interneurons in the PFC.
 
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Publishing Date
2010-02-24
 
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