Efeito do sistema nervoso autônomo simpático ß-adrenérgico sobre a expressão do gene do GLUT4 no jejum.

Alves, Ana Barbara Teixeira

doi:10.11606/D.42.2007.tde-18102007-093539

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Master's Dissertation

DOI

https://doi.org/10.11606/D.42.2007.tde-18102007-093539

Document

Master's Dissertation

Author

Alves, Ana Barbara Teixeira (Catálogo USP)

Full name

Ana Barbara Teixeira Alves

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Human Physiology

Date of Defense

2007-07-24

Published

São Paulo, 2007

Supervisor

Machado, Ubiratan Fabres (Catálogo USP)

Committee

Machado, Ubiratan Fabres (President)
Rossoni, Luciana Venturini
Zanella, Maria Teresa

Title in Portuguese

Efeito do sistema nervoso autônomo simpático ß-adrenérgico sobre a expressão do gene do GLUT4 no jejum.

Keywords in Portuguese

GLUT4
Jejum
Músculo esquelético
Sistema nervoso autônomo simpático beta-adrenérgico.

Abstract in Portuguese

O estudo investigou a participação do sistema nervoso simpático ß-adrenérgico sobre a regulação da expressão gene do GLUT4 em músculo vermelho (sóleo) e branco (EDL) no jejum. Foram estudados ratos alimentados livremente, jejuados por 48 horas e sob bloqueio ß1/ß2 ou bloqueio ß1/ß2/ß3 ou sob estímulo ß1/ß2/ß3-adrenérgicos. Os músculos sóleo e EDL foram analisados por Northern e Western Blotting. O jejum diminuiu o peso dos animais, a glicemia, a insulinemia, e aumentou concentração de ácidos graxos livres (AGL). O bloqueio ß1/ß2 aumentou a insulinemia, o bloqueio ß1/ß2/ß3 reduziu a glicemia e o estímulo ß1/ß2/ß3 aumentou AGL. O jejum aumentou mRNA do GLUT4 no sóleo e EDL, os bloqueios ß-adrenérgicos reduziram o mRNA no sóleo e EDL, e o estímulo ß aumentou o mRNA somente no sóleo. O jejum aumentou a proteína GLUT4 no sóleo e nos tratamentos somente reduziu com o bloqueio triplo, no EDL. O jejum aumentou a expressão do gene do GLUT4 em músculo esquelético. A atividade simpática ß-adrenérgica é fundamental para aumentar a expressão do GLUT4 no sóleo e manter no EDL.

Title in English

Effect of ß-adrenergic sympathetic autonomic nervous system on GLUT4 gene expression on fasting.

Keywords in English

Fasting
GLUT4
Skeletal muscle
ß-adrenergic sympathetic autonomic nervous system .

Abstract in English

The study investigated the participation of ß-adrenergic sympathetic nervous system in the regulation of GLUT4 gene expression in red (soleus) and white (EDL) muscles during fasting. Rats were fasted for 48hours and treated with ß1/ß2-adrenoceptors antagonist or ß1/ß2/ß3-adrenoceptors antagonist or ß1/ß2/ß3-adrenoceptors agonist. Soleus and EDL muscles were analyzed by Northern and Western Blotting. Fasting decreased body weight, glycemia, insulinemia, and increased non esterified free fatty acids (NEFA). The insulinemia increased with ß1/ß2 blockade, glycemia decreased with triple blockade, NEFA increased with ß stimulus. The GLUT4 mRNA increased in soleus and EDL after fasting, and it decreased in soleus and EDL after ß1/ß2 blockade and triple blockade, and increased in soleus after ß stimulus. GLUT4 protein increased in soleus of fasted rats, and decreased with triple blockade in EDL. Fasting increased GLUT4 gene expresion in skeletal muscle. The ß-adrenergic sympathetic activity is essencial to increase GLUT4 expression in soleus and to preserve it in EDL.

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Publishing Date

2007-10-22

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