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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2015.tde-02062015-131022
Document
Author
Full name
Rafael Vianna Croffi
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Nunes, Maria Tereza (President)
Akamine, Eliana Hiromi
Chaves, Maria Luiza Morais Barreto de
Porto, Catarina Segreti
Seraphim, Patricia Monteiro
Title in Portuguese
Bases moleculares das ações rápidas do T3 na captação de glicose em célula adiposa 3T3-L1.
Keywords in Portuguese
Ação não-genômica
Adipócitos
Captação de glicose
Células 3T3-L1
Hormônio tireoidiano
Vias de sinalização intracelulares
Abstract in Portuguese
Os hormônios tireoidianos atuam sobre o metabolismo dos diversos tecidos do organismo e participam da regulação do consumo de glicose pelas células. Estudos já evidenciaram que o T3 atua, dependendo do tipo celular, aumentando a expressão de algumas isoformas dos transportadores de glicose (GLUTs) e a translocação do GLUT4 para a membrana plasmática, melhorando, também, a captação de glicose (CGlic) em poucos minutos. Os mecanismos envolvidos nessas ações do T3, contudo, ainda não estão bem esclarecidos. O objetivo do presente estudo foi investigar as possíveis vias de sinalização envolvidas na ação aguda do T3 sobre a CGlic em células adiposas 3T3-L1. Nossos dados demonstraram que o T3 promove aumento na CGlic, com pico aos 10 min, retornando ao nível do controle após 30 min de incubação das células com o hormônio. Sugerimos que essa ação depende, ao menos, de duas vias de sinalização. Uma delas envolve a ativação das proteínas Src, PI3K e Akt. A outra, aparentemente, é iniciada a partir da membrana plasmática via integrina aVb3
Title in English
Molecular basis of rapid T3 actions on glucose uptake in 3T3-L1 adipocytes.
Keywords in English
3T3-L1 cells
Adipocytes
Glucose uptake
Intracellular signaling pathways
Non-genomic action
Thyroid hormone
Abstract in English
Thyroid hormones act on the metabolism of many tissues and participate in the regulation of glucose consumption by cells. Studies from this and other laboratories have demonstrated in muscle and adipose cells that T3 increases, in a short period (minutes), the expression of some glucose transporter (GLUTs) isoforms and GLUT4 translocation to the plasma membrane leading to an improvement of glucose uptake. However, the mechanisms involved in these T3 actions are still not clear. The aim of this study was to investigate the possible signaling pathways involved in the acute T3 action on glucose uptake in 3T3-L1 adipocytes. Our results have shown that T3 increases glucose uptake with a peak at 10 min returning to the control level after 30 min of the cell incubation with the hormone. We suggest that this action depends on at least two parallel signaling pathways. One involves the activation of Src, PI3K and Akt proteins, while the other involves another mechanism triggered by T3, apparently, from the plasma membrane at aVb3 integrin.
 
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Publishing Date
2015-06-03
 
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