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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2014.tde-13082014-170430
Document
Author
Full name
Nathalia Molina
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Otton, Rosemari (President)
Munhoz, Carolina Demarchi
Pires, Adriana Cristina Levada
Title in Portuguese
Avaliação de parâmetros oxidativos e funcionais em linfócitos de ratos obesos suplementados cronicamente com extrato de chá verde.
Keywords in Portuguese
Catequinas
Chá verde
Dieta hipercalórica
Linfócitos
Obesidade
Sistema imunológico
Abstract in Portuguese
O chá verde (CV) é um fitoterápico produzido a partir da planta Camellia sinensis e apresenta propriedades antioxidantes, anti-inflamatórias, anticancerígenas e antiobesidade. Foi realizado um estudo para investigar se o CV seria capaz de modular alguns parâmetros funcionais de linfócitos de animais induzidos à obesidade por dieta hipercalórica. Os grupos suplementados receberam 500 mg/kg de CV durante 22 dias seguida da dieta hipercalórica por mais 44 dias acompanhada também do tratamento com o CV. O tratamento com o CV reduziu a proliferação celular, a atividade das enzimas metabólicas hexoquinase e glicose-6-fosfato desidrogenase, aumentou a atividade de enzimas antioxidantes, diminuiu a produção de espécies reativas de oxigênio, a produção de citocinas pró- inflamatórias (IL-6, IL-1β, TNF-α) e restaurou os índices de IL-10 que se encontrava diminuído no grupo obeso. Os efeitos observados pelo CV podem proporcionar um ambiente mais adequado para o bom funcionamento celular, diminuindo a produção de mediadores inflamatórios que estão presente na obesidade.
Title in English
Evaluation of oxidative and functional parameters in lymphocytes of obese rats chronically supplemented with green tea extract.
Keywords in English
Catechins
Green tea
Hypercaloric diet
Immune system
Lymphocytes
Obesity
Abstract in English
Green tea (GT) is a phytochemical produced from Camellia sinensis and it has anti-inflammatory, anti-cancer, anti-obesity and antioxidant properties. This study was conducted to investigate whether the GT was able to modulate some lymphocyte functional parameters of obesity - induced animals by hypercaloric diet. Supplemented groups received 500 mg/kg GT for 22 days followed by hypercaloric diet for additional 44 days, together with the GT treatment. Treatment with GT reduced the cell proliferation capacity, the activity of metabolic enzymes hexokinase and glucose-6-phosphate dehydrogenase, increased the activity of antioxidant enzymes, decreased the production of reactive oxygen species, production of proinflammatory cytokines (IL-6, IL-1β, TNF-α) and restored the levels of IL-10 which was reduced by obesity. The GT effects can provide a more suitable environment for proper lymphocyte function, also reducing production of inflammatory mediators in obesity.
 
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Publishing Date
2014-08-14
 
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