Ocorrência de patógenos intestinais e fatores de risco associados à infecção entre os índios tapirapé habitantes da Amazônia Mato-Grossense, Brasil.

Malheiros, Antonio Francisco

doi:10.11606/T.42.2012.tde-28052012-093605

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DOI

https://doi.org/10.11606/T.42.2012.tde-28052012-093605

Document

Doctoral Thesis

Author

Malheiros, Antonio Francisco (Catálogo USP)

Full name

Antonio Francisco Malheiros

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Biology of Host-Pathogen Interactions

Date of Defense

2012-02-02

Published

São Paulo, 2012

Supervisor

Shaw, Jeffrey Jon (Catálogo USP)

Committee

Shaw, Jeffrey Jon (President)
Alfieri, Silvia Celina
Dias, Ricardo Augusto
Gryschek, Ronaldo Cesar Borges
Pinto, Pedro Luiz Silva

Title in Portuguese

Ocorrência de patógenos intestinais e fatores de risco associados à infecção entre os índios tapirapé habitantes da Amazônia Mato-Grossense, Brasil.

Keywords in Portuguese

Biologia molecular
Doenças parasitárias
Epidemiologia
Helmintologia
Protozoologia
Saúde indígena

Abstract in Portuguese

A prevalência de patógenos intestinais foi estudada entre os índios da etnia Tapirapé, da Amazônia mato-grossense, por meio de técnicas coproparasitológicas, imunológicas e moleculares. Do total de 1526 amostras, 83,35% apresentaram ao menos um parasito intestinal e 65% tinham mais de um parasito (poliparasitismo). Entamoeba coli foi o mais prevalente (827/1526 - 54,19%). Entamoeba histolytica/dispar (581/1526 - 38,07%), Giardia intestinalis (287/1526 - 18,81%), Blastocystis spp. (257/1526 - 16,84%) e Ancylostoma spp. (293/1526 - 19,20%) também foram freqüentes. Cistos de Giardia intestinalis foram seqüenciados utilizando os genes b-Giardina e gdh. Apenas os assemblages A e B foram encontrados, sendo que o assemblage A foi o mais prevalente. Análise molecular de Blastocystis spp. demonstrou que, por meio do gene SSU-rNA, o subtipo 1 foi o mais dominante entre os Tapirapé, seguido pelos subtipos 2 e 3. Com base nisso, G. intestinalis e Blastocystis spp. são potencialmente zoonóticos. Os resultados corroboram com outros estudos realizados na Amazônia brasileira.

Title in English

The occurrence of intestinal pathogens and risk factors associated with their infection among the Tapirapé indians of the Amazon region of Mato Grosso, Brazil.

Keywords in English

Epidemiology
Helminthology
Indian health
Molecular biology
Parasitic diseases
Protozoology

Abstract in English

The prevalence of intestinal pathogens was studied in indigenous of the Tapirapé ethnic from Amazon region of Mato Grosso State, using the coproparasitological, immunological and molecular. Of the total 1,526 fecal samples 83.35% had at least one intestinal parasite and 65% had more than one parasite (poliparasitism). The most prevalent parasite was Entamoeba coli (827/1526 - 54.19%). Entamoeba histolytica/dispar (581/1526 - 38, 07%), Giardia intestinalis (287/1526 - 18.81%), Blastocystis spp. (257/1526 - 16.84%) and Ancylostoma spp. (293/1526 -19.20%) were found too. Cysts of G. intestinalis were sequence by b-Giardina and GDH gene. Only assemblages A and B were found and assemblage A was the most prevalent. The molecular characterization of Blastocystis spp. by SSU-rRNA demonstrated that subtype 1 was dominant followed by subtypes 2 and 3. So, G. intestinalis and Blastocystis spp. are potentially zoonotic. The results are in agreement with previous studies conducted in the Brazilian Amazon.

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Publishing Date

2012-06-04

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