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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2018.tde-19022018-104850
Document
Author
Full name
Vivian Aparecida Ramos Petersen
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Rocha, Lincoln Suesdek (President)
Alves, João Marcelo Pereira
Klaczko, Louis Bernard
Marino, Celso Luis
Marrelli, Mauro Toledo
Title in Portuguese
Polimorfismo em Aedes scapularis: caracterização genética e morfológica de um dos vetores de filárias e arbovírus.
Keywords in Portuguese
Aedes scapularis
COI
Complexo de espécies
DNA mitocondrial completo
Morfometria geométrica
Polimorfismo
Abstract in Portuguese
A presente tese compreende 2 capítulos, dedicados à investigação de complexo de espécie e polimorfismo em Aedes scapularis. No Capítulo 1 foi desenvolvido um resumo técnico dos procedimentos utilizados na análise e montagem dos genes do DNA mitocondrial de 19 indivíduos da espécie Aedes scapularis e de como foi realizada a filtragem do DNA de alguns mosquitos em relação ao DNA amplificado do animal picado. No Capítulo 2 realizamos as análises de complexo de espécies e polimorfismo molecular em Aedes scapularis, com espécimes da Mata Atlântica, Caatinga e Mata Amazônica utilizando os marcadores: Morfometria Geométrica, gene Citocromo Oxidase Subunidade I e análise do DNA mitocondrial completo. Encontramos alto polimorfismo evidenciados por todos os marcadores populacionais nos mosquitos dos diferentes biomas estudados e alta estruturação populacional na análise filogenética dos genes mitocondriais codificadores de proteínas. Obtivemos duas conclusões majoritárias em nosso trabalho: 1) a espécie Aedes scapularis é genética e morfologicamente polimórfica em todos os ambientes estudados 2) Não encontramos evidência de complexo de espécies neste mosquito e então acreditamos que este mosquito trate-se de uma única entidade taxonômica.
Title in English
Polymorphisms in Aedes scapularis: Genetic and morphological characterization of one filaria and arbovirus vector.
Keywords in English
COI
Geometric morphometrics
Polymorphism
Species complex
Whole mitochondrial DNA
Abstract in English
The present thesis comprises 2 chapters, dedicated to the investigation of species complex and polymorphism in Aedes scapularis. In Chapter 1 a technical summary of procedures used in the analysis and assembly of mitochondrial DNA genes of 19 individuals of the species Aedes scapularis and how the "filtering" of the DNA of some mosquitoes was performed in relation to the amplified DNA of the animal bitten. In Chapter 2 we performed the analyzes of species complex and molecular polymorphism in Aedes scapularis, with Atlantic Forest, Caatinga (Xerophytic sparse tropical scrub) and Amazon Forest specimens using the markers: geometric morphometrics, Cytochrome Oxidase Subunit I gene Whole mitochondrial DNA analysis. We found high polymorphism evidenced for every markers in the mosquitoes of different biomes studied and high population structure in the phylogenetic analysis of the protein-coding mitochondrial genes. We obtained two principal conclusions in our work: 1) the species Aedes scapularis is genetic and morphologically polymorphic in all studied environments 2) We did not find evidence of species complex in this mosquito and therefore we believe that this mosquito is a single taxonomic entity.
 
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Publishing Date
2018-02-19
 
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