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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2010.tde-17012011-131013
Document
Author
Full name
Mariana Devicari
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Rocha, Lincoln Suesdek (President)
Ribolla, Paulo Eduardo Martins
Sallum, Maria Anice Mureb
Title in Portuguese
Caracterização populacional de Aedes scapularis (Diptera; Culicidae): aspectos moleculares, morfofuncionais e morfológicos.
Keywords in Portuguese
Aedes
DNA Mitocondrial
Evolução
Insetos vetores
Morfometria
Abstract in Portuguese
A espécie Aedes scapularis é um dos culicídeos de grande importância médica. Está distribuída nas Américas e tem grande competência vetora para diversos arbovírus. No estado de São Paulo, há ocorrência de Ae. scapularis em vários municípios, como em Pariquera-Açu e São Paulo. O objetivo desse trabalho foi testar se há diferenciação genética - morfológica entre essas populações, podendo diagnosticar existência de espécies crípticas em Aedes scapularis. As populações estudadas foram Pariquera-Açu (PAR), Parque Ecológico do Tietê em São Paulo (PET) e Butantã (BUT). Os parâmetros utilizados foram: Morfometria geométrica da asa (forma e tamanho), estudo do gene mitocondrial COI, análise dos espaçadores internos transcritos ITS2 e análise morfológica de ovos. Com os resultados obtidos, podemos concluir que a divergência populacional é atestada por padrões geográficos de forma alar, gene mitocondrial COI e razão comprimento e largura dos ovos, mas extensões de estudos em outras áreas precisam ser feitos para poder atestar espécies crípticas em Aedes scapularis.
Title in English
Characterization population of Aedes scapularis (Diptera: Culicidae): aspect molecular, morphological and morphometric.
Keywords in English
Aedes
DNA Mitochondrial
Evolution
Insect Vectors
Morphometry
Abstract in English
The species Aedes scapularis is a culicidae of medical importance. It is distributed in the Americas and has a high vector competence for many arboviruses. In state of São Paulo, have occurrence of Ae. scapularis in many cities, such as Pariquera-Acu and the city of São Paulo. The aim of this study was to determine differentiation genetic- morphology among these populations, being able to diagnose the existence of cryptic species in Aedes scapularis. The populations studied were Pariquera-Acu (PAR), the Tietê Ecological Park in Sao Paulo (PET) and Butantã (BUT). The parameters used were: wing geometric morphometry (shape and size), study of mitochondrial gene COI, analysis of internal transcribed spacers ITS2 and morphological analysis of eggs. With these results, we conclude that divergence population is attested by the geographical patterns of wing shape, and COI mitochondrial gene length and width ratio of eggs, but extensions of studies in other areas need to be made in order to attest cryptic species in Aedes scapularis.
 
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Publishing Date
2011-08-08
 
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