Mecanismo de geração de resposta imune humoral induzida pelo direcionamento do antígeno MSP119PADRE para duas populações distintas de células dendríticas via receptores DEC205 e DCIR2.

Sulczewski, Fernando Bandeira

doi:10.11606/D.42.2018.tde-12032018-153708

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Master's Dissertation

DOI

https://doi.org/10.11606/D.42.2018.tde-12032018-153708

Document

Master's Dissertation

Author

Sulczewski, Fernando Bandeira (Catálogo USP)

Full name

Fernando Bandeira Sulczewski

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Biology of Host-Pathogen Interactions

Date of Defense

2017-11-24

Published

São Paulo, 2017

Supervisor

Boscardin, Silvia Beatriz (Catálogo USP)
Rosa, Daniela Santoro - (Co-supervisor) (Catálogo USP)

Committee

Boscardin, Silvia Beatriz (President)
Barbuto, Jose Alexandre Marzagao
Keller, Alexandre de Castro
Lima, Maria Regina D'Imperio

Title in Portuguese

Mecanismo de geração de resposta imune humoral induzida pelo direcionamento do antígeno MSP1₁₉PADRE para duas populações distintas de células dendríticas via receptores DEC205 e DCIR2.

Keywords in Portuguese

Células dendríticas
Direcionamento de antígenos
Resposta imune celular
Resposta imune humoral
Subtipos de DCs

Abstract in Portuguese

As células dendríticas (DCs) são células do sistema imune inato que são especializadas na instrução de repostas imunes adaptativas No baço murinho, as DCs convencionais podem ser classificadas em CD8α⁺ DEC205⁺ e CD8α^- DCIR2⁺. Anticorpos monoclonais (mAbs) αDEC205 e αDCIR2 (33D1) conjugados a proteínas antigênicas e são utilizados como estratégia de direcionamento de antígenos para as DCs CD8α⁺ e CD8α^-. Foi utilizado o antígeno quimérico MSP1₁₉PADRE conjugado aos mAbs αDEC205 e αDCIR2 e o Poly I:C como adjuvante. A montagem da resposta celular e humoral foi analisada 3, 4, 5 e 6 dias após primeira e a segunda As DCS CD8α^- são especializadas na instrução de células T_FH. Porém, num segundo dose de mAbs há a instrução de uma resposta Th2/Th17. E, o direcionamento de antígenos para DCS CD8α⁺ induz uma resposta Th1, indicando que essas células são especializadas na instrução desse perfil de resposta auxiliar. Apesar disso, na segunda imunização há a diferenciação de células T_FH.

Title in English

Mechanism of generation of humoral immune response induced by targeting of MSP1₁₉PADRE antigen to two different subsets of dendritic cells via DEC205 and DCIR2 receptors.

Keywords in English

Antigen targeting
Cellular immune response
DC subsets
Dendritic cell
Humoral immune response

Abstract in English

As dendritic cells (DC) are innate immune cells that are specialized to prime adaptive immune cells. In the murine spleen, conventional DCs can be classified into CD8α⁺ DEC205+ and CD8α^-DCIR2⁺. Monoclonal antibodies (mAbs) αDEC205 and αDCIR2 (33D1) conjugated to antigenic proteins have benn used as antigen targeting strategy to CD8α⁺ and CD8α^- DCs. MSP1₁₉PADRE chimeric antigen conjugated to mAbs αDEC205 and αDCIR2 and Poly I: C as adjuvant was used. The assembly of the cellular and humoral response was analyzed 3, 4, 5 and 6 days after the first and second doses of imnunization. DCs CD8α^- are specialized in the instruction of T_FH cells. However, there is an a promotion of Th1 response by CD8α⁺, indicating that these cells are specialized in the instruction of the helper response profile. Nevertheless, in the second immunization there is a differentiation of T_FH cells.

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FernandoBandeiraSulczewski_Mestrado_I.pdf (4.55 Mbytes)

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Publishing Date

2018-03-12

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