Diversidade, biologia, filogeografia e taxonomia molecular de tripanossomas de anuros da família Leptodactylidae.

Sato, Lyslaine Hatsue

doi:10.11606/D.42.2015.tde-07102015-145210

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Master's Dissertation

DOI

https://doi.org/10.11606/D.42.2015.tde-07102015-145210

Document

Master's Dissertation

Author

Sato, Lyslaine Hatsue (Catálogo USP)

Full name

Lyslaine Hatsue Sato

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Biology of Host-Pathogen Interactions

Date of Defense

2015-06-12

Published

São Paulo, 2015

Supervisor

Teixeira, Marta Maria Geraldes (Catálogo USP)

Committee

Teixeira, Marta Maria Geraldes (President)
Jared, Carlos Alberto Goncalves Silva
Silva, Reinaldo José da

Title in Portuguese

Diversidade, biologia, filogeografia e taxonomia molecular de tripanossomas de anuros da família Leptodactylidae.

Keywords in Portuguese

Anfíbios
Anura
Biogeografia
Filogeografia
Hemoprotozoários
Morfologia
Neotrópico
Rãs
Taxonomia molecular
Trypanosoma

Abstract in Portuguese

A fim de avaliar a diversidade genética, morfológica e filogeografia de tripanossomas de leptodactilídeos, analisamos diversos isolados encontrados em amostras de sangue e fígados obtidos no Brasil (Amazônia, Caatinga, Cerrado, Mata Atlântica e Pantanal), na Colômbia e na Venezuela. Inferências filogenéticas baseadas em marcadores moleculares (V7V8 SSUrDNA e gGAPDH) demonstraram uma complexa associação entre clados, biomas e hospedeiros vertebrados. Nossos resultados corroboram a monofilia dos trypanossomas de anuros e sugerem a existência de, ao menos, seis clados (An01-06) nesse grupo com 12 candidatos a novas espécies. O clado An01 compreende tripanossomas de leptodactilídeos e hilídeos de todos os biomas. Os clados An02, An05 e An06 são formados, predominantemente, por tripanossomas de leptodactlídeos de diferentes biomas brasileiros, da Colômbia e da Venezuela. O clado An03 é formado por tripanossomas de leptodactilídeos, bufonídeos e flebotomíneos, todos do bioma amazônico. O clado An04 agrupa apenas tripanossoma exóticos da América do Norte, África e Europa.

Title in English

Diversity, biology, phylogeography and molecular taxonomy of trypanosomes of frogs in the Leptodactylidae Family.

Keywords in English

Amphibians
Anura
Biogeography
Frogs
Hemoprotozoans
Molecular taxonomy
Morphology
Neotropics
Phylogeny
Trypanosoma

Abstract in English

Aiming to investigate the genetic diversity, the morphology and the phylogeny of leptodactylid trypanosomes, we analyzed a number of trypanosomes from blood and liver samples from Brazil (Amazonia, Caatinga, Cerrado, Atlantic Forest and Pantanal), Colombia and Venezuela. Phylogenetic analyses based on molecular markers (V7V8 SSUrDNA and gGAPDH) demonstrated a complex association between clades, biomes and vertebrate hosts. Our results corroborated the monophyly of anuran trypanosomes and suggested the existence of, at least, six clades (An01-06) inside the anuran clade with 12 candidate new species. Clade An01 groups trypanosomes from leptodactylids and hylids collected in all biomes. Clades An02, An05 and An06 are composed, mostly, by leptodactylid trypanosomes from several Brazilian biomes, Colombia and Venezuela. Clade An03 aggregates parasites from leptodactylids, bufonids and phlebotomines from Amazonia. Clade An04 groups only exotic trypanosome species from North America, Africa and Europe.

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Publishing Date

2015-10-08

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