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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2014.tde-25112014-103358
Document
Author
Full name
Leandro Nogueira Pressinotti
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Silva, Jose Roberto Machado Cunha da (President)
Blazquez, Francisco Javier Hernandez
Knöbl, Terezinha
Paiva, Maria José Tavares Ranzani de
Seelaender, Marilia Cerqueira Leite
Title in Portuguese
Influência da temperatura na cicatrização do tegumento de jacaré-do-pantanal Caiman yacare (DAUDIN, 1802).
Keywords in Portuguese
Crocodiliano
Inflamação
Pele
Regeneração
Temperatura
Abstract in Portuguese
Os crocodilianos são animais ectotermos passíveis de cultivo, na zootecnia, onde ocorrem diversas mutilações de apêndices inteiros, ou mesmo a formação de feridas. Nesse sentido, há a necessidade de compreender como a temperatura interfere nas respostas de restituição tecidual desses organismos. Os Caiman yacare foram submetidos à retirada cirúrgica de um osteodermo e mantidos sob 23 ºC e 33 ºC, 8 jacarés em cada temperatura. Posteriormente, os jacarés tinham suas lesões fotografadas para análise macroscópica. Amostras foram fixadas processadas e coradas para histoquímica e imunohistoquímica. Macroscopicamente foi observada a manutenção do coágulo até 30 dias sob 23 ºC, enquanto a cicatriz já estava reepitelizada sob 33 ºC. Microscopicamente foi observado o atraso da migração da epiderme, escassez de fibras e de novos vasos na derme sob 23 ºC. Nesse sentido, recomendamos o isolamento dos animais mutilados em um tanque com controle de temperatura para agilizar o fechamento epidérmico.
Title in English
Influence of temperature on the wound healing of the integument of the jacaré-do-pantanal Caiman yacare (Daudin, 1801).
Keywords in English
Crocodilians
Inflammation
Regeneration
Skin
Temperature
Abstract in English
Crocodilians are ectothermic animals capable of to be farming, where there are multiple appendages mutilation or even the formation of wounds. In this sense, becomes necessary to understand how temperature affects the responses of tissue restitution of these organisms. The Caiman yacare had an osteoderm surgically removed and the caimans were kept at 23 º C and 33 º C, 8 caimans at each temperature. Subsequently, the caimans had their injuries photographed for macroscopic analysis. Samples were fixed and processed for histochemistry and immunohistochemistry staining. Macroscopically the clot were maintained until 30 days at 23 ° C, while the scar was already re-epithelized at 33 º C. Microscopically, were observed the delayed migration of epidermis, diminishing of fibers and new vessels in dermis at 23 ° C. Accordingly, we recommend the isolation of animals mutilated in a tank with temperature control to accelerate the epidermal closure.
 
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Publishing Date
2014-11-27
 
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