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Doctoral Thesis
DOI
10.11606/T.42.2011.tde-21072011-134443
Document
Author
Full name
Jonatas Bussador do Amaral
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Santelli, Glaucia Maria Machado (President)
Borella, Maria Ines
Chavez, Victor Elias Arana
Engler, Silvya Stuchi Maria
Ionta, Marisa
Title in Portuguese
Células MCF-7 como modelo 3D no estudo de câncer de mama humano.
Keywords in Portuguese
Apoptose
Células cultivadas de tumor
Citoesqueleto
Esferóides multicelulares
Neoplasias mamárias
Terceira dimensão
Abstract in Portuguese
O diferencial da cultura de células em 3-dimensões é permitir que as células explorem as 3-dimensões do espaço, aumentando assim as interações com o ambiente e entre as células. Em estudos relacionados à biologia do câncer de mama, vem ganhando espaço a utilização de esferóides para estudos que visam à compreensão da morfogênese do espaço luminal. Neste trabalho foi mostrado que as células MCF-7 reorganizam-se em estruturas tubulares e acinares. Em ambas as situações, a formação do lúmen veio acompanhada pelo estabelecimento de uma camada de células polarizadas, arranjo este muito semelhante ao encontrado em glândulas mamárias. Os resultados apresentados apontam para a existência de uma população de células na linhagem MCF-7 que não estão totalmente comprometidas ao fenótipo tumoral. Mantidos diferenciados, os esferóides de células MCF-7 apontam como um novo modelo para estudos relacionados à formação do lúmen, permitindo assim explorar o papel de diferentes vias como as relacionadas a apoptose, autofagia, diferenciação e sobrevivência celular.
Title in English
MCF-7 cells as a 3d model in the study of human breast cancer.
Keywords in English
Apoptosis
Breast neoplasms
Cultured tumor cells
Cytoskeleton
Multicelular Spheroids
Third Dimension
Abstract in English
As a particularity, a 3D cell culture permits cells to explore the three dimensions of the space thereby increasing cell-cell interactions, as well as interaction with the environment. In studies related to breast cancer biology, spheroids are becoming widely used in the aim to comprehend luminal space morphogenesis. We showed that MCF-7 cells reorganize themselves in tubular and acinar structures. In both situations, lumen formation was accompanied by the establishment of a layer of polarized cells, an arrangement that is very similar to that of breast glands. The presented results suggest the existence of an MCF cell line population not completely committed to the tumor phenotype. When maintained as differentiated, MCF-7 cell spheroids can be a new model for studies regarding lumen formation, thereby exploring the role of diiferent pathways, such as those related to cell apoptosis, autophagy, differentiation and survival.
 
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Publishing Date
2011-08-10
 
WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.
  • BUSSADOR DO AMARAL, Jônatas, e MACHADO-SANTELLI, Gláucia Maria. A cultura de células em 3 dimensões e a sua aplicação em estudos relacionados a formação do lúmen. Naturalia [online], 2011, vol. 34, p. 1-20. Artigo de Revisão. [acesso 2011-09-27]. Disponível em : <http://www.periodicos.rc.biblioteca.unesp.br/index.php/naturalia/article/viewFile/4399/3600>
  • BUSSADOR DO AMARAL, Jônatas, SHINITI URABAYASHI, Marcel, and MARIA MACHADO‑SANTELLI, Gláucia. Cell death and lumen formation in spheroids of MCF-7 cells [doi:10.1042/CBI20090024]. Cell Biology International [online], 2010, vol. 34, n. 3, p. 267-274.
  • DO AMARAL, Jônatas Bussador, et al. MCF-7 Cells as a Three-Dimensional Model for the Study of Human Breast Cancer [doi:10.1089/ten.tec.2011.0260]. Tissue Engineering Part C: Methods [online], 2011, p. 1109010655.
  • BUSSADOR DO AMARAL, Jônatas, et al. Cell death and lumen formation in spheroids of MCF-7 cells. In Gordon Research Conference- Mammary Gland, Lucca, 2010.
  • BUSSADOR DO AMARAL, Jônatas, et al. Formation of Spheroids in Mamary Adenocarcinoma MCF-7 cells. In Applied Cancer Research, São Paulo, 2005. São Paulo : Applied Cancer Research, 2005. vol. 2, ISBN 18085539.
  • BUSSADOR DO AMARAL, Jônatas, et al. MCF-7 cells as a 3D model in the study of human breast cancer. In Histology and Histopatology, Granada, 2011. Granada : Histology and Histopatology, 2011. vol. 26, n. 1, p. 342. ISBN 0213-3911.
  • BUSSADOR DO AMARAL, Jônatas, and MACHADO-SANTELLI, Gláucia Maria. Ultrastructure of Adenocarcinoma Mammary Cells in 3D cell culture. In XXI CONGRESSO DA SOCIEDADE BRASILEIRA DE MICROSCOPIA E MICROANÁLISE XXI CSBMM: multidisciplinaridade, Búzios, 2007.
  • BUSSADOR DO AMARAL, Jônatas, e MACHADO-SANTELLI, Gláucia Maria. The end of the tunnel: MCF-7 cells death and proliferation inside spheroids. In XIV Congresso da Sociedade Brasileira de Biologia Celular, São Paulo, 2008.
  • BUSSADOR DO AMARAL, Jônatas, MORAES FREITAS, Vanessa, e MACHADO-SANTELLI, Gláucia Maria. A utilização de linhagem de adenocarcinoma mamário (MCF-7) para o estudo da morfogênese luminal. In XVIII Congresso Brasileiro de Cancerologia, Curitiba, 2009.
  • BUSSADOR DO AMARAL, Jônatas, MORAIS FREITAS, Vanessa, and MACHADO-SANTELLI, Gláucia Maria. Survival and Death in 3D Cell Culture. In The 17th IFSM International Microscopy Congress, Rio de Janeiro, 2010.
  • URABAYASHI, Marcel, BUSSADOR DO AMARAL, Jônatas, and MACHADO-SANTELLI, Jônatas. Growing Spheroids of Mammary Adenocarcinoma MCF-7 Cells In vitro: Morphological Aspects. In The American Society for Cell Biology: 46th Annual Meetting, San Diego, 2006.
  • URABAYASHI, Marcel, BUSSADOR DO AMARAL, Jônatas, e MACHADO-SANTELLI, Gláucia Maria. Apoptosis increasing is associated with the in vitro spheroids development. In Brazilian Symposium on Extracellular Matrix and International Symposium on Extracellular Matrix (SIMEC), Búzios, 2006.
  • BUSSADOR DO AMARAL, Jônatas, and MACHADO-SANTELLI, Gláucia Maria. 3D cell culture (breast cancer cell line- MCF 7) São Paulo, 2011. Filme. online. [cited 2011-09-27]. Available from : <http://www.youtube.com/watch?v=2B8miQ5ImDI>
  • BUSSADOR DO AMARAL, Jônatas, and MACHADO-SANTELLI, Gláucia Maria. Cancer cells in 3D cell culture (breast adenocarcinoma) São Paulo, 2009. Filme. online. [cited 2011-09-27]. Available from : <http://www.youtube.com/watch?v=upOUApWno6E>
  • BUSSADOR DO AMARAL, Jônatas, and MACHADO-SANTELLI, Gláucia Maria. Image of Distinction-Nikon Small World 2011 São Paulo, 2011. Outros. online. [cited 2011-09-27]. Available from : <http://www.nikonsmallworld.com/detail/year/2011/43>
  • BUSSADOR DO AMARAL, Jônatas, MODIA JúNIOR, Roberto Cabado, and MACHADO-SANTELLI, Gláucia Maria. Cover of Tissue Engineering, Part C: Methods : November 2011 New Rochelle : Mary Ann Liebert, Inc publishers, 2011. Outros.
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