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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2007.tde-28082008-160245
Document
Author
Full name
Oyama Rodrigues da Silva
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2007
Supervisor
Committee
Rivera, Irma Nelly Gutierrez (President)
Ferreira, Antônio José Piantino
Nunes, Iolanda Aparecida
Sato, Maria Inês Zanoli
Timenetsky, Jorge
Title in Portuguese
Modelo teórico de estimativa de risco de Salmonella Enteritidis em sistema integrado de produção de frango de corte e tipagem molecular de Salmonella spp. oriundas de aves e rações submetidas a diferentes tratamentos com ácido
Keywords in Portuguese
Avicultura
Biologia molecular
Frangos de corte
Microbiologia
Salmonella Typhimurium
Abstract in Portuguese
O presente trabalho objetivou identificar os fatores de risco para a presença de S. Enteritidis no sistema de produção de frangos de corte, avaliar, qualificar e quantificar as variáveis encontradas e elaborar um modelo teórico de estimativa de risco deste sorovar em frangos criados em sistema de integração. Os dados foram obtidos de trabalhos recentes realizados por alguns autores e deram subsídios à realização de uma análise de riscos microbiológicos. Para caracterização molecular foram utilizadas 42 cepas de Salmonella isoladas de frangos e rações inoculados experimentalmente com uma cepa de S. Typhimurium. A inoculação da bactéria foi realizada na ração e a mesma tratada com diferentes concentrações dos ácidos propílico, fórmico e acético sendo, então, fornecida para consumo ad libitum até os 21 dias de idade, quando as aves foram sacrificadas. Foram obtidos diferentes perfis genéticos com o uso do ERIC e BOX-PCR, que se mostraram eficientes para discriminação das cepas em estudo.
Title in English
Theoretical model of risk assessment of Salmonella Enteritidis in broiler chicken production integrated system and molecular typing of Salmonella spp. from birds and feed submitted to decontamination with different organic acids.
Keywords in English
Chicken-cut
Poultry
Risk Assessment
Salmonella Enteritidi
Salmonella Typhimuirum
Abstract in English
The aim of this work was identify the risk factors for S. Enteritidis in the production system of broiler chickens, to evaluate, qualify and quantify the variables studied and to make a theoretical model of risk assessment of this serovar in broilers in integration system. Therefore, the data was obtain from works of some authors and supported the proposed model of microbiological risk analysis. For molecular characterization were included 42 Salmonella spp. strains isolated from chicks and feed experimentally inoculated with S. Tiphimurium. After inoculation of feed with the specific dose of strain, it was submitted to treatment with propilic, formic and acetic acids in several concentrations and it was given to birds ad libitum until 21 days old, when they were sacrificed. It was obtained different patterns through the ERIC and BOX-PCR techniques, which showed good discrimination power for the strains analyzed.
 
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Publishing Date
2008-08-28
 
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