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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2014.tde-27062014-171721
Document
Author
Full name
Natália Galdi Quel
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2013
Supervisor
Committee
Yano, Tomomasa (President)
Knöbl, Terezinha
Martins, Luciano Moura
Title in Portuguese
Escherichia coli Vacuolating Factor (ECVF) como fator associado a celulite aviária.
Keywords in Portuguese
Escherichia coli Patogênica Aviária (APEC)
Escherichia coli Vacuolating Factor (ECVF)
Celulite aviária
Abstract in Portuguese
E. coli isoladas de lesões de celulite aviária em frangos de corte produzem uma citotoxina, denominada ECVF (E. coli Vacuolating Factor), que causa intensa vacuolização citoplasmática em células aviárias, mas não em células mamárias. A importância de ECVF na patogenia da celulite foi avaliada neste estudo. ECVF purificado foi inoculado subcutaneamente em frangos de corte, e induziu sinais de inflamação nos tecidos subcutâneo, adiposo e conjuntivo. Em ensaios de citotoxicidade, foi verificado que ECVF induz alterações citoplasmáticas e nucleares que podem afetar diretamente o metabolismo celular, entre elas condensação da cromatina e fragmentação nuclear, intensa vacuolização citoplasmática e desorganização do citoesqueleto, conduzindo à apoptose. Também foi verificada interação de ECVF com proteínas de células aviárias, em detrimento das de células de mamíferos, sugerindo uma especificidade da toxina a este tipo celular. Nossos resultados, apoiados por dados de estudos anteriores, permitem sugerir um importante papel de ECVF na patogenia da celulite aviária.
Title in English
Escherichia coli Vacoulating Factor (ECVF) as a factor associated to avian cellulitis.
Keywords in English
Escherichia coli Vacoulating Factor (ECVF)
Avian cellulitis
Avian Pathogenic Escherichia coli (APEC)
Abstract in English
E. coli isolated from cellulitis lesions in broiler chickens produce a citotoxin, called ECVF (Escherichia coli Vacuolating Factor), which causes intense cytoplasm vacuolization in avian cells, but not in mammalian cells. The importance of ECVF in the pathogenesis of avian cellulitis was assessed in this study. Purified ECVF was inoculated subcutaneously in broiler chickens, and induced signs of inflammation on subcutaneous, adipose and connective tissues. In citotoxicity assays, we verified that ECVF induced cytoplasmic and nuclear alterations, which can affect cellular metabolism directly, such as chromatin condensation and nuclear fragmentation, intense cytoplasm vacuolization, and disorganization of cytoskeleton, leading to apoptosis. It was also verified the interaction of ECVF with proteins of avian cells, instead of those from mammalian cells, suggesting the specificity of this toxin to this cells. Our results, supported by data from previous studies, suggest an important role of ECVF in the pathogenesis of avian cellulitis.
 
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Publishing Date
2014-07-02
 
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