Pesquisa e caracterização de amostras de ExPEC (Extraintestinal Pathogenic Escherichia coli ) isoladas de infecções do trato urinário (ITU) de cães e gatos.

Osugui, Lika

doi:10.11606/D.42.2008.tde-27032009-162026

Home

Facilities

Master's Dissertation

DOI

https://doi.org/10.11606/D.42.2008.tde-27032009-162026

Document

Master's Dissertation

Author

Osugui, Lika (Catálogo USP)

Full name

Lika Osugui

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Microbiology

Date of Defense

2008-12-10

Published

São Paulo, 2008

Supervisor

Castro, Antonio Fernando Pestana de (Catálogo USP)

Committee

Castro, Antonio Fernando Pestana de (President)
Amaral, Tania Aparecida Tardelli Gomes do
Piazza, Roxane Maria Fontes

Title in Portuguese

Pesquisa e caracterização de amostras de ExPEC ("Extraintestinal Pathogenic Escherichia coli ") isoladas de infecções do trato urinário (ITU) de cães e gatos.

Keywords in Portuguese

Cães e gatos
ExPEC (Extraintestinal Pathogenic Escherichia coli
Fatores de virulência
Grupos filogenéticos de Escherichia coli
Infecções do trato urinário (ITU)
UPEC (Uropathogenic \"Escherichia coli\")

Abstract in Portuguese

As ITU são as mais freqüentes infecções ocasionadas por ExPEC. Entre os fatores de virulência (FV) encontram-se nestas cepas adesinas, invasinas, toxinas, sideróforos, e evasinas, localizados em plasmídios ou ilhas de patogenecidade. O objetivo deste estudo foi caracterizar 45 cepas de E. coli isoladas de 33 cães e 7 gatos com ITU, quanto aos sorotipos, FV e grupos filogenéticos. Dos sorogrupos relacionados às ITU foram encontrados O6 (20%), O2 (16%), O25 (4%), O4 e O11 (4% cada um). Entre os genes pesquisados, foram encontrados fimH (100%), pap (47%), sfa (33%) e iha (4%); ibeA (29%); cnf1 (31%), hlyA (27%); fyuA (80%), iucD (22%); traT (51%); cvaC (20%) e malX (67%). Os isolados felinos foram agrupados em B2 (89%) e D (11%), enquanto os caninos em A (5,5%), B1 (19,5%), B2 (55,5%) e D (19,5%). Estes resultados sugerem que as ExPEC isoladas de cães e gatos apresentam potencial patogênico para ocasionar doenças mais graves que as ITU, assim como ocorre em humanos. Além disso, a similitude com as amostras humanas reforça a hipótese acerca de seu potencial zoonótico.

Title in English

Characterization of ExPEC ("Extraintestinal Pathogenic Escherichia coli") isolated from dogs and cats with uinary tract infections (UTI).

Keywords in English

E. coli phylogenetic groups
Dogs and cats
ExPEC(Extraintestinal Pathogenic Escherichia coli)
UPEC (Uropathogenic Escherichia coli)
Urinary tract infections
Virulence factors

Abstract in English

The ability of ExPEC to cause extraintestinal infections in humans, dogs, and cats is associated with the expression of a variety of virulence factors (VF). The aim of this study was to evaluate the frequency of VF related to ExPEC, serotypes, and phylogenetic groups in 45 strains isolated from 33 dogs and 7 cats with UTI. These strains presented serogroups related with extraintestinal infections, e.g. O6 (20%), O2 (16%), O25 (4%), O4 e O11 (each one) and the following genes: fimH (100%), pap (47%), sfa (33%) e iha (4%); ibeA (29%); cnf1 (31%), hlyA (27%); fyuA (80%), iucD (22%); traT (51%); cvaC (20%) e malX (67%), cvaC (20%), and malX (67%). All feline strains were concentrated in B2 (89%) and D (11%) phylogenetic groups, whereas the canine ones were distributed in the four groups, A (5,5%), B1 (19,5%), B2 (55,5%) and D (19,5%). These findings suggesting that ExPEC isolated from dog and cat contain virulence markers to cause diseases, more severe than UTI, likewise in humans. Besides, these the close similarity between human and animal ExPEC supports the hypotesis of zoonotic potencial of them.

WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.

LikaOsugui_Mestrado.pdf (1.89 Mbytes)

Publishing Date

2009-04-06

Derived works

WARNING: Learn what derived works are clicking here.