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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2011.tde-26012012-140354
Document
Author
Full name
Fabiane Lucy Ferreira Castro
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Correa, Benedito (President)
Fernandes, Andrezza Maria
Gambale, Valderez
Potenza, Marcos Roberto
Sabino, Myrna
Title in Portuguese
Interação entre fungos toxigênicos (Aspergillus flavus e Fusarium verticillioides) e carunchos (Sitophilus zeamais) em amostras de grãos de milho.
Keywords in Portuguese
Aflotoxinas
Fungos
Fungos toxicogênicos
Microtoxinas
Milho
Abstract in Portuguese
Foi pesquisada a habilidade de carunchos Sitophilus zeamais em veicular esporos de Aspergillus flavus e Fusarium verticillioides e a conseqüente produção de micotoxinas. Grãos de milho foram mantidos em frascos conectados por uma mangueira formando um sistema fechado (lados A e B) e divididos em seis grupos: G1 (milho + caruncho - Lado A); G2 (Milho + A. flavus - Lado A); G3 (milho + A. flavus + caruncho - Lado A); G4 (milho + F. verticillioides - Lado A), G5 (milho + F. verticillioides + caruncho - Lado A) e G6 (milho + A. flavus + F. verticillioides + caruncho - Lado A). O lado B continha grãos estéreis. Após 10, 20 e 30 dias de incubação foram realizadas: pesagem, atividade de água, microbiota fúngica, determinação de micotoxinas, análise nutricional, microscopia eletrônica de varredura e PCR-RT em tempo real. Frente aos resultados obtidos constata-se a importância do Sitophillus zeamais como vetor de fungos e a importância de boas práticas de manipulação e armazenamento de grãos, visando reduzir os riscos de contaminação e deterioração.
Title in English
Interaction between toxigenic fungus (Aspergillus flavus e Fusarium verticilliodes) and weevils (Sitophilus zeamais) in samples of maize grains.
Keywords in English
Aflatoxin
Corn
Fungi
Mycotoxins
Toxigenic fungi
Abstract in English
The weevils Sitophilus zeamais ability was examined to propagate spores of Aspergillus flavus and Fusarium verticillioides and the production of mycotoxins. Corn grains was conserved in flasks connected by a rubber to form a closed system (side A and B) and divided in six groups: G1 (corn + weevil - side A); G2 (corn + A. flavus - side A); G3 (corn + A. flavus + weevil - side A); G4 (corn + F. verticillioides - side A), G5 (corn + F. verticillioides + weevil - side A) e G6 (corn + A. flavus + F. verticillioides + weevil - side A). The side B contained sterile grains. After 10, 20 and 30 days of incubation were realized: weighing, activity water, mycoflora, determination of mycotoxins, nutritional analysis, scanning electron microscope and Real time PCR-RT. In front of the results was observed the importance of Sitophilus zeamais like a fungus vector and the importance of Good Manufacturing Practices and Stores of grains, to reduce the risks of contamination and deterioration.
 
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Publishing Date
2012-03-20
 
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