Fungemia por leveduras: perfis fenotípicos e moleculares e sensibilidade antifúngica de amostras isoladas no hospital das clínicas de Botucatu, São Paulo.

Ruiz, Luciana da Silva

doi:10.11606/T.42.2008.tde-25112008-103426

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DOI

https://doi.org/10.11606/T.42.2008.tde-25112008-103426

Document

Doctoral Thesis

Author

Ruiz, Luciana da Silva (Catálogo USP)

Full name

Luciana da Silva Ruiz

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Microbiology

Date of Defense

2008-10-29

Published

São Paulo, 2008

Supervisor

Paula, Claudete Rodrigues (Catálogo USP)

Committee

Paula, Claudete Rodrigues (President)
Cury, Arlete Emily
Durigon, Edison Luiz
Gambale, Valderez
Szeszs, Maria Walderez

Title in Portuguese

Fungemia por leveduras: perfis fenotípicos e moleculares e sensibilidade antifúngica de amostras isoladas no hospital das clínicas de Botucatu, São Paulo.

Keywords in Portuguese

Candida
Fungemia
Micologia aplicada
Micologia médica
Perfil molecular
Sensibilidade antifúngica

Abstract in Portuguese

Os objetivos deste estudo foram: re-identificar 70 isolados de Candida em micoteca por método tradicional e API 20C; identificar a presença de C. dubliniensis; determinar e comparar as concentrações inibitórias mínimas (CIMs) das amostras, frente a sete antifúngicos (E-test); caracterizar molecularmente as amostras seqüenciais de Candida pela técnica de PFGE; estudar e comparar o perfil genotípico de isolados de C. albicans e C. parapsilosis, através da análise de marcadores microsatélites e cariotipagem. O nível de concordância entre o método tradicional e o API 20C foi de 93%. Nenhum isolado de C. dubliniensis foi identificado no estudo. Em relação à sensibilidade antifúngica, a maioria das amostras apresentou alta porcentagem de sensibilidade às sete drogas testadas. A análise molecular pela técnica de PFGE, mostrou seis perfis de cariótipos diferentes em 24 amostras seqüenciais. Em relação à comparação das técnicas de PFGE e microssatélites, observamos que a última mostrou maior poder discriminatório para as amostras de estudadas.

Title in English

Fungemia by yeasts: molecular and phenotypic profiles and susceptibility to antifungal agents of samples isolated at hospital das clínicas de Botucatu, São Paulo, Brazil.

Keywords in English

Candida
Antifungal susceptibility
Fungemia
Molecular profile
Mycology applied

Abstract in English

This study was aimed to: identify the 70 isolates of Candida in a culture collection by the traditional method and by API 20C; identify the presence of C. dubliniensis; determine and compare the minimum inhibitory concentrations (MICs) of these samples in regard to the seven drugs (E-test); molecularly characterize sequential samples from the same patient by the PFGE technique; study the genotypic profile of all the isolates of C. albicans and C. parapsilosis by means of the microsatellite technique and compare the results with those obtained by the PFGE technique. The level of agreement between the traditional method and the API 20C was 93%. No isolate of C. dubliniensis was identified in the study. In relation to antifungal susceptibility, most of the samples presented a high percentage of susceptibility to the seven drugs tested. The molecular analysis by the PFGE technique revealed six different karyotype profiles among 24 sequential samples. In the comparison between the PFGE and microsatellite, the latter showed a greater discriminatory power for samples.

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Publishing Date

2009-05-13

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