Análise bacteriológica de infecções pulpares em dentes decíduos.

Fabris, Antonio Scalco

doi:10.11606/T.42.2011.tde-20102011-162418

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Doctoral Thesis

DOI

https://doi.org/10.11606/T.42.2011.tde-20102011-162418

Document

Doctoral Thesis

Author

Fabris, Antonio Scalco (Catálogo USP)

Full name

Antonio Scalco Fabris

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Microbiology

Date of Defense

2011-05-16

Published

São Paulo, 2011

Supervisor

Campos, Mario Julio Avila (Catálogo USP)

Committee

Campos, Mario Julio Avila (President)
Fraga, Mariana Minatel Braga
Imparato, Jose Carlos Pettorossi
Pereira, Rosana de Souza
Simionato, Maria Regina Lorenzetti

Title in Portuguese

Análise bacteriológica de infecções pulpares em dentes decíduos.

Keywords in Portuguese

Dente decíduo
Infecções bacterianas
Polpa dentária
Reação em cadeia por polimerase

Abstract in Portuguese

Foram analisados dentes decíduos com cárie dental profunda de 110 crianças, sendo coletadas 103 amostras de polpa necrosada e 7 de fístulas gengivais. Morfotipos bacterianos foram visualizados pelas colorações de Gram e Brenn-Brown, e os DNA foram obtidos e usados na detecção bacteriana por PCR. A predominância de cocos Gram positivos (81,8%) e cocobacilos Gram negativos (49,1%) foram observadas. Em 88 amostras de polpas, microrganismos com maior ocorrência foram: Enterococcus spp. (50%), P. gingivalis (49%), F. nucleatum (25%) e P. nigrescens (11,4%). Foram detectados em fístulas: P. gingivalis (43%), Enterococcus spp. (28,6%), F. nucleatum (14,3%), P. nigrescens (14,3%), e D. pneumosintes (14,3%). Os nossos resultados permitem concluir que a microbiota envolvida nas infecções pulpares em dentes decíduos é similar em termos qualitativos àquela observada em dentes permanentes. Entretanto, a predominância de Enterococcus spp. e P. gingivalis deve ser levado em consideração pelos clínicos em casos necessários de tratamento endodôntico em crianças com dentição decídua.

Title in English

Bacteriological analysis of pulp infection in deciduous teeth.

Keywords in English

Bacterial infections
Deciduous tooth
Dental pulp
Polymerase chain reaction

Abstract in English

In this study, deciduous teeth with deep caries from 110 children, with 103 pulp necrosis and 7 gingival fistula samples were evaluated. Bacterial morphotypes were visualized by Gram staining and Brown-Brenn. DNA were obtained and used in bacterial detection by using PCR. The predominance of Gram-positive cocci (81.8%) and Gram-negative coccobacilli (49.1%) were observed. In 88 pulp samples a high frequency of microrganisms were observed: Enterococcus spp. (50%), P. gingivalis (49%), F. nucleatum (25%) and P. nigrescens (11.4%). In fistulas were detected: P. gingivalis (43%), Enterococcus spp. (28.6%), F. nucleatum (14.3%), P. nigrescens (14.3%), and Dialister pneumosintes (14.3%). Our results, suggest that the involved microbiota in pulp infections of deciduous teeth are qualitatively similar than those permanent teeth. However, a predominance of Enterococcus spp. and P. gingivalis was observed, and it must be considered in the endodontic treatment in children with primary dentition.

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Publishing Date

2011-11-11

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