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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2011.tde-20032012-163902
Document
Author
Full name
Vanessa Kummer Perinazzo de Oliveira
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2011
Supervisor
Committee
Paula, Claudete Rodrigues (President)
Correa, Benedito
Krebs, Vera Lúcia Jornada
Title in Portuguese
Ocorrência das espécies de leveduras isoladas de sangue e cateter de pacientes internados em Hospital Público Infantil de São Paulo (período 2007 a 2010).
Keywords in Portuguese
Candida albicans
Candida
Candidemia
Candidíase
Infecção hospitalar
Leveduras
Abstract in Portuguese
Investigamos a ocorrência de espécies de leveduras isoladas em um hospital infantil. Pesquisamos a presença de C. dubliniensis; investigamos as espécies do complexo Candida parapsilosis e estudamos as espécies deste complexo quanto a produção de biofilme. Pesquisamos os fatores relacionados a virulência e o perfil de sensibilidade pelo método Etest®. Dentre os anos em estudo C. albicans foi a levedura mais isolada com 36.45% seguida de C. tropicalis 23.36%, C. parapsilosis sensu stricto 21.49%, Pichia anômala 5.61%, C. guilliermondii 4.67%, C. krusei 2.80%; C. orthopsilosis 1.87%, C. glabrata 1.87%, C. metapsilosis 0.94%, C. pararugosa 0.94%. C. dubliniensis não foi isolada nesta pesquisa. No estudo do complexo Candida parapsilosis, C. parapsilosis sensu estricto foi a espécie mais prevalente. Produziram biofilme apenas oito amostras de C. parapsilosis sensu estricto e uma amostra de C. orthopsilosis. As espécies não-albicans produziram mais proteinase e hialuronidase do que C. albicans. No estudo da fosfolipase e hemolisina, C. albicans foi a espécie mais produtora. Todas as amostras foram sensíveis para anfotericina B e para fluconazol quatro amostras foram resistentes. Duas amostras foram resistentes para caspofungina.
Title in English
Yeasts species isolated from blood and catheter from patients admitted into Children's Public Hospital of São Paulo (period 2007 to 2010).
Keywords in English
Candida albicans
Candida
Candidemia
Candidiasis
Hospital infection
Yeast
Abstract in English
We investigated the occurrence of yeast species isolated in a public hospital. We researched the presence of C. dubliniensis and investigated the species within the complex Candida parapsilosis and studied this complex in relation to biofilm´s production. We also investigated the factors related to virulence and studied the sensibility profile by the "Etest ®" method. During the years of study, C. albicans was the most isolated yeast with 36.45% followed by C. tropicalis 23,36%, C. parapsilosis sensu stricto 21,49%, Pichia anomala 5,61%, C. guilliermondii 4.67%, C. krusei 2.80%, C. orthopsilosis 1.87%, C. glabrata 1.87%, C. metapsilosis 0.94%, C. pararugosa 0.94%. C. dubliniensis was not isolated in this study. In the study of Candida parapsilosis complex, C. parapsilosis sensu stricto was the most prevalent species. Only eight strains of C. parapsilosis sensu stricto and one strain of C. orthopsilosis produce biofilm. The non-albicans species showed greater activity of the proteinase and hyaluronidase than C. albicans. In the study of hemolysin and phospholipase, C. albicans was the most producer. All strains were susceptible to amphotericin B and to fluconazole four were resistant. For caspofungin, two strains were resistant.
 
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Publishing Date
2012-05-25
 
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