Estudo de genes de Caulobacter crescentus importantes para a sobrevivência em baixas temperaturas.

Mazzon, Ricardo Ruiz

doi:10.11606/T.42.2011.tde-20032012-162702

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DOI

https://doi.org/10.11606/T.42.2011.tde-20032012-162702

Document

Doctoral Thesis

Author

Mazzon, Ricardo Ruiz (Catálogo USP)

Full name

Ricardo Ruiz Mazzon

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Microbiology

Date of Defense

2011-11-21

Published

São Paulo, 2011

Supervisor

Marques, Marilis do Valle (Catálogo USP)

Committee

Marques, Marilis do Valle (President)
Baldini, Regina Lúcia
Galhardo, Rodrigo da Silva
Oliveira, Carla Columbano de
Silva, Rosa Maria

Title in Portuguese

Estudo de genes de Caulobacter crescentus importantes para a sobrevivência em baixas temperaturas.

Keywords in Portuguese

Caulobacter crescentus
Genética bacteriana
Genética microbiana
Genética molecular

Abstract in Portuguese

Caulobacter crescentus sobrevive em baixas temperaturas e mostrou ser um organismo psicrotolerante e de alta resistência ao congelamento, característica resultante de múltiplos fatores. C. crescentus possui quatro genes codificantes para CSPs, sendo cspA e cspB induzidos em baixas temperaturas e cspB, cspC e cspD em fase estacionária. A ausência de cspA e cspB ou cspA e cspC confere grande deficiência de crescimento em baixas temperaturas. cspA e cspB não são autorregulados e são regulados pós-transcricionalmente via estabilização de seu mRNA e traducionalmente após o choque-frio. A expressão de cspB é influênciada por CspC a 30 graus e durante o choque-frio, e por CspC, SpdR e SpoT durante a fase estacionária. A ausência de CspC ou CspC e CspD compromete a adaptação à fase estacionária promovendo alterações morfológicas. Nenhuma das CSPs de C. crescentus é capaz de reverter o fenótipo de E. coli BX04 por expressão heteróloga, embora todas possuam atividade antiterminadora que, nestas proteínas, não depende dos mesmos aminoácidos que CspE de E. coli.

Title in English

Study of Caulobacter crescentus genes important to low temperature survival.

Keywords in English

Caulobacter crescentus
Bacterial genetics
Microbial genetics
Molecular genetics

Abstract in English

Characterization of Caulobacter crescentus cold response was performed. This bacterium showed to be psicrotolerant and have remarkable freezing resistance, which may be a result of multiple traits. C. crescentus has four CSP encoding genes, being cspA and cspB cold-induced and cspB, cspC and cspD stationary phase-induced. The absence of cspA and cspB or cspA and cspC led to growth deficiency under low temperature incubation. cspA and cspB are not self-regulated and are post-transcriptionally and translationally regulated during cold-shock. The cspB gene expression is affected by CspC at exponential growth phase and by CspC, SpdR and SpoT at stationary phase. The absence of CspC, or CspC and CspD, affects stationary phase fitness of this organism, also promoting morphological alterations. None of the C. crescentus CSPs were able to restore the phenotype of E. coli BX04 strain by heterologous expression. Although all of them have shown to be transcription antiterminators, this ability is not dependent on the same critical aminoacids displayed by CspE from E. coli.

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Publishing Date

2012-05-25

Derived works

WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.

MAZZON, R. R., et al. Cold shock genes cspA and cspB from Caulobacter crescentus are post-transcriptionally regulated and important for cold adaptation [doi:10.1128/JB.01422-12]. Journal of Bacteriology [online], 2012, vol. 194, p. 6507-6517.
Mazzon, Ricardo R., et al. Characterization of Caulobacter crescentus response to low temperature and identification of genes involved in freezing resistance [doi:10.1111/j.1574-6968.2008.01337.x]. FEMS Microbiology Letters [online], 2008, vol. 288, p. 178-185.
MARQUES, MV., et al. Characterization of Caulobacter crescentus mutants for cold shock genes. In 108th General Meeting of American Society for Microbiology, Boston, 2008. Abstracts of 108th General Meeting of American Society for Microbiology., 2008. Abstract.
MARQUES, MV., MAZZON, R. R., e Elza A. S. Lang. Identification of genes important for Caulobacter crescentus resistance to freezing. In 11th International Symposium on Microbial Ecology, Viena, 2006. Book of Abstracts., 2006. Resumo.
MAZZON, R. R., et al. Caracterização de dois genes de Caulobacter crescentus importantes para a sobrevivência em baixa temperatura. In 53o Congresso Brasileiro de Genética, Águas de Lindóia, 2007. Resumos do 53o Congresso Brasileiro de Genética., 2007. Resumo.
MAZZON, R. R., et al. Characterization of Mutants for Cold Shock Genes in Caulobacter crescentus. In 109th Meeting of the American Society for Microbiology, Philadelphia, 2009. Abstracts of 109th Meeting of the American Society for Microbiology., 2009. Abstract.
MAZZON, R. R., et al. Role and regulation of Caulobacter crescentus csp paralogues in response to stress. In Molecular Genetics of Bacteria & Phages, Cold spring Harbor, NY, 2010. Abstracts of Molecular Genetics of Bacteria & Phages., 2010. Abstract.
MAZZON, R. R., et al. Role and regulation of cold shock proteins in Caulobacter crescentus. In FEMS 2011- 4th Congress of European Microbiologists, Genebra, 2011. Abstracts of FEMS 2011- 4th Congress of European Microbiologists., 2011. Abstract.
MAZZON, R. R., et al. Role and regulation of proteins containing Cold Shock Domains in Caulobacter crescentus. In 3rd Caulobacter Meeting, Boston, 2009. Abstracts of the 3rd Caulobacter Meeting., 2009. Abstract.
MAZZON, R. R., e MARQUES, MV. Identificação e análise fenotípica de mutantes de Caulobacter crescentus sensíveis a congelamento. In 13o Simpósio Internacional de Iniciação Científica, Ribeirão Preto, 2005. Resumos do 13o Simpósio Internacional de Iniciação Científica., 2005. Resumo.
Mazzon, Ricardo R., et al. . RNA metabolism and the cold shock response in Caulobacter. In 4th Caulobacter Meeting, Montreal, 2012. Abstracts of 4th Caulobacter Meeting., 2012. Abstract.
Mazzon, Ricardo R., et al. Obtenção e análise fenotípica de mutantes de genes codificantes de proteínas contendo domínios de choque frio de Caulobacter crescentus. In 54º Congresso Brasileiro de Genética, Salvador, 2008. Resumos do 54º Congresso Brasileiro de Genética., 2008. Resumo.
Mazzon, Ricardo R., et al. Post-transcriptional regulation of Caulobacter crescentus genes encoding cold shock proteins. In 4th ASM Conference on Prokaryotic Development, Montreal, 2012. Abstracts of 4th ASM Conference on Prokaryotic Development., 2012. Abstract.
Mazzon, Ricardo R., et al. RNA metabolism and the cold shock response in Caulobacter. In FEBS RNA workshop, Tavira, Portugal, 2012. Abstracts of . FEBS RNA workshop., 2012. Resumo.
orientadora de Ricardo Ruiz Mazzon, finalista do Prêmio Jovem Geneticista - Sociedade Brasileira de Genética