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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2010.tde-19052010-160451
Document
Author
Full name
Gislaine Gomes Martins Guethi
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2010
Supervisor
Committee
Rivera, Irma Nelly Gutierrez (President)
Almeida, Sandro Rogerio de
Cury, Arlete Emily
Melhem, Marcia de Souza Carvalho
Pellizari, Vivian Helena
Title in Portuguese
Caracterização de leveduras do gênero Trichosporon isoladas de três regiões costeiras do Estado de São Paulo
Keywords in Portuguese
Trichosporon spp
Água de mar
Antifúngicos
Areia
Leveduras
Sequenciamento
Abstract in Portuguese
As regiões costeiras estão sendo ameaçadas por apresentarem uma exploração desordenada e predatória de seus recursos naturais alterando a biodiversidade. Leveduras fazem parte do ecossistema marinho, entretanto algumas espécies estão associadas à micoses superficial, subcutânea e/ou sistêmicas. O objetivo deste estudo foi quantificar leveduras, isolar e caracterizar Trichosporon spp., em amostras água de mar e areia em três regiões costeiras do estado de São Paulo: Baixada Santista, Canal de São Sebastião e Ubatuba. Foram coletadas 126 amostras de água de mar e areia. As áreas foram caracterizadas usando parâmetros microbiológicos. A contagem de leveduras variou de <1 a 1.300UFC/100mL em água de mar e <1 a 3.200UFC/g em areia. As leveduras isoladas foram identificadas usando a metodologia tradicional e testes genotípicos. De um total de 102, 30,5% foi confirmado pelo API ID 32C, 40,2% pelo auxanograma e 63,7% pelo PCR. Os isolados identificados pelo PCR foram seqüenciados e 41,5% foram Trichosporon spp.
Title in English
Characterization of Trichosporon spp. Isolated from three coastal regions of São Paulo State, Brazil
Keywords in English
Trichosporon spp
Antifungal
Sand
Sea water
Sequencing
Yeasts
Abstract in English
Coastal regions are being threatened because they have a predatory and uncontrolled exploitation of natural resources by changing biodiversity. Yeasts are part of the marine ecosystem, though some species are associated with superficial mycoses, subcutaneous and / or systemic. The objective of this study was to quantify yeast, isolation and Trichosporon spp. In samples of sea water and sand in three regions of the state of São Paulo: Santos, Canal de São Sebastião and Ubatuba. We collected 126 samples of sea water and sand. The areas were characterized by microbiological parameters. Yeast counts ranged from <1 to 1.300UFC/100mL in sea water and <1 to 3.200UFC / g in sand. The yeasts were identified using the traditional methods and genotypic tests. From a total of 102, 30.5% was confirmed by API ID 32C, by 40.2% and 63.7% auxanograma PCR. The isolates identified by PCR were sequenced and 41.5% were Trichosporon spp.
 
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Publishing Date
2010-06-08
 
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