Utilização de peptídeo sintético (P10) associado ao tratamento com drogas antifúngicas no controle da paracoccidioidomicose experimental

Marques, Alexandre Ferreira

doi:10.11606/T.42.2007.tde-17102007-183226

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DOI

https://doi.org/10.11606/T.42.2007.tde-17102007-183226

Document

Doctoral Thesis

Author

Marques, Alexandre Ferreira (Catálogo USP)

Full name

Alexandre Ferreira Marques

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Microbiology

Date of Defense

2007-08-17

Published

São Paulo, 2007

Supervisor

Taborda, Carlos Pelleschi (Catálogo USP)

Committee

Taborda, Carlos Pelleschi (President)
Almeida, Sandro Rogerio de
Benard, Gil
Puccia, Rosana
Travassos, Luiz Rodolpho Raja Gabaglia

Title in Portuguese

Utilização de peptídeo sintético (P10) associado ao tratamento com drogas antifúngicas no controle da paracoccidioidomicose experimental

Keywords in Portuguese

Adjuvantes imunológicos
Anergia
Antifúngicos
P. brasiliensis
Paracoccidioidomicose
Peptídeo sintético

Abstract in Portuguese

A paracoccidioidomicose (PCM), doença sistêmica de caráter granulomatoso, causada pelo fungo termodimórfico Paracoccidioides brasiliensis. A PCM é endêmica na América Latina e atinge principalmente indivíduos do sexo masculino com atividades econômica ligada a agricultura. Os pacientes com PCM exigem tratamento a base de sulfametoxazol/trimetoprim, anfotericina B, e derivados azólicos por longos períodos. A gp43, possui 416 aminoácidos, onde um trecho específico de 15 aminoácidos (QTLIAIHTLAIRYAN) designado como (P10), é reconhecido pelos linfócitos T de camundongos e humanos. No presente estudo avaliamos o efeito aditivo da imunização do P10 com às drogas antifúngicas utilizadas no tratamento da PCM. Nossos resultados indicam um efeito aditivo entre a imunização com P10 e o tratamento medicamentoso em camundongos Balb/c infectados. Associado a redução significativa da carga fúngica no pulmão, baço e fígado desses animais, detectamos aumento dos níveis de IL-12 e IFN-? e diminuição de IL-4 e IL-10.

Title in English

Use of synthetic peptide (P10) associate with antifungal drugs to the treatment in the control of experimental paracoccidioidomyicosis.

Keywords in English

adjuvants
anergy
P. brasiliensis
P10
paracoccidioidomycosis
sulfamethoxazole-trimethoprim
treatment.

Abstract in English

Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by Paracoccidioides brasiliensis, a thermal dimorphic fungus. PCM is endemic in Latin America affecting mainly rural workers. The patients with PCM demand treatment the base of sulfametoxazole/trimethoprim, amphotericin B and derivatives azolic. The gp43 has 416-mer and mice and human T lymphocytes are stimulated by a 15-mer peptide designated as P10 (QTLIAIHTLAIRYAN). In the present work we evaluated the additive effect of P10 immunization and antifungal drugs utilizing in PCM treatment. Ours results showed an additive protective effect between immunization with P10 and drugs treatment with infected Balb/c mice. Associated to the reduction of fungal burden in the lung, spleen and liver we observed increase of levels of IL-12 and IFN-? and reduction of IL-4 and IL-10.

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Publishing Date

2007-10-17

Derived works

WARNING: The material described below relates to works resulting from this thesis or dissertation. The contents of these works are the author's responsibility.

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