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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2018.tde-16042018-095257
Document
Author
Full name
Celio Roberto Jonck
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Pellizari, Vivian Helena (President)
Gomez, José Gregorio Cabrera
Hoffmann, Christian
Silva, Aline Maria da
Sluys, Marie Anne van
Title in Portuguese
Ecologia microbiana de riachos influenciados pela exploração de petróleo no Brasil.
Keywords in Portuguese
Comunidades microbianas
Diversidade taxonômica
Indicadores ecológicos
Indústria do petróleo
Potencial funcional
Abstract in Portuguese
Atividades humanas podem causar degradação e impedir que serviços ambientais cheguem á sociedade. Evitá-la demanda constante monitoramento, que pode ser realizado com indicadores ambientais. Buscamos selecionar indicadores da exploração de petróleo nas comunidades microbianas de riachos da Amazônia, Mata Atlântica, Cerrado e Caatinga. Avaliamos as diversidades taxonômica (Sequenciamento 16S) e funcional (GeoChip 5.0M) e comparamos os resultados de amostras de água, sedimento, biofilme e solo das margens de riachos influenciados por campos de petróleo com áreas de referência. Todas as estações apresentaram alta riqueza, baixa similaridade taxonômica e alta similaridade funcional. Impactos da atividade de exploração de petróleo foram relacionados ao uso do solo e não à contaminação por hidrocarbonetos, já que as estações não tinham histórico de vazamentos. Os indicadores identificados não foram atreldos a grupos taxonômicos ou atividades metabólicas específicas, sendo mais promissora a utilização de um conceito baseado em sequencias genéticas indicadoras.
Title in English
Microbial ecology of streams influenced by petroleum exploration in Brazil.
Keywords in English
Ecological indicators
Functional diversity
Microbial communities
Petroleum industry
Taxonomic diversity
Abstract in English
Human activities can cause degradation and prevent environmental services from reaching society. Avoid it demand constant monitoring, which can be carried out with environmental indicators. We sought to select indicators of oil exploration in the microbial communities of streams in the Amazon, Atlantic Forest, Cerrado and Caatinga morphoclimatic domains. We evaluated taxonomic (16S Sequencing) and functional (GeoChip 5.0M) diversity and compared the results from water, sediment, biofilm and soil (from the margins of streams) samples influenced by oil fields with reference areas. All of them presented high richness, low taxonomic similarity and high functional similarity. Impacts of the oil exploration activity are related to the use of soil and not to hydrocarbon contamination, since the sampling stations we choose are historically not affected by oil spills. The indicators we identified are not related to taxonomic groups or specific metabolic activities, and the use of a concept based on indicator sequences is more promising.
 
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Publishing Date
2018-04-17
 
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