• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2014.tde-15122014-131506
Document
Author
Full name
Gabriela Martins Reis
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Correa, Benedito (President)
Gonçalves, Sarah Santos
Ishida, Kelly
Sabino, Myrna
Telles, Evelise Oliveira
Title in Portuguese
Distribuição de fungos e fumonisinas e expressão dos genes FUM em grãos de milho transgênico da semeadura à colheita.
Keywords in Portuguese
Fusarium verticillioides
Fases de amadurecimento do grão
Fumonisinas
Genes FUM
Milho transgênico
Abstract in Portuguese
O objetivo foi avaliar a expressão dos genes FUM como indicativo da contaminação por Fusarium verticillioides e por fumonisinas nos grãos de milho transgênico durante todo o amadurecimento dos grãos. Constatou-se contaminação fúngica em 100% das amostras. As frequências de Fusarium foram de 3,85% a 100%, sendo 97% F. verticillioides. Foi detectada FB1 em 100% das amostras e FB2 em 18,41%. Os níveis de FB1 variaram de 0,02 mg/g a 5,12 mg/g e FB2 de níveis não detectáveis a 1,74 mg/g. Todos os genes FUM foram amplificados em 33,8% das amostras, e as expressões foram em média entre 3,88 e 7,65 (valores obtidos considerando a amostra calibradora). Não houve correlação positiva entre a porcentagem de F. verticillioides, FB1 e FB2 e os fatores abióticos (p>0,05), o mesmo ocorreu entre a expressão relativa dos genes FUM e os percentuais de isolamento de F. verticillioides e os níveis de FB1 e FB2 (p>0,05), e constatou-se correlação positiva entre a probabilidade de amplificação dos genes FUM e a fase de amadurecimento do grão (p<0,05).
Title in English
Distribution of fungi and fumonisins and FUM genes expression in transgenic corn grains from sowing to harvest.
Keywords in English
FUM genes cluster
Fusarium verticillioides
Fumonisins
Kernel maturation stages
Transgenic corn
Abstract in English
This study evaluated the FUM gene expression in corn grains from sowing to harvest as evidence of Fusarium verticillioides contamination and fumonisins production. All samples (100%) were contaminated with fungi. The isolation frequencies of Fusarium genre ranged from 3,85 % to 100 % and 97 % were F. verticillioides. FB1 was detected in 100% of the samples and FB2 in 18,41 %. The levels of FB1 ranged from 0,02 mg/g to 5,12 mg/g, while FB2 ranged from undetectable levels to 1,74 mg/g. All FUM genes were amplified in 33,8 % of the samples, with average ranging from 3.88 to 7,65, when compared to the calibrator sample. Positive correlation among the F. verticillioides isolation percentage, FB1 e FB2 production and the abiotic factors was not detected (p>0,05), positive correlation was also not detected among the FUM gene cluster relative expressions, F. verticillioides isolation percentage and FB1 and FB2 production (p>0,05). However, a positive correlation was detected between the probability of FUM genes expression and the kernel maturity (p<0,05).
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2014-12-16
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2024. All rights reserved.