Avaliação de alguns microrganismos da microbiota intestinal endógena de crianças eutróficas com sobrepeso e obesas em idade escolar.

Silva, Aline Ignacio Silvestre da

doi:10.11606/D.42.2014.tde-12082014-170007

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Master's Dissertation

DOI

https://doi.org/10.11606/D.42.2014.tde-12082014-170007

Document

Master's Dissertation

Author

Silva, Aline Ignacio Silvestre da (Catálogo USP)

Full name

Aline Ignacio Silvestre da Silva

Institute/School/College

Instituto de Ciências Biomédicas

Knowledge Area

Microbiology

Date of Defense

2014-04-16

Published

São Paulo, 2014

Supervisor

Nakano, Viviane (Catálogo USP)

Committee

Nakano, Viviane (President)
Elias Junior, Waldir Pereira
Silva Filho, Luiz Vicente Ribeiro Ferreira da

Title in Portuguese

Avaliação de alguns microrganismos da microbiota intestinal endógena de crianças eutróficas com sobrepeso e obesas em idade escolar.

Keywords in Portuguese

Bacteroides
Clostridium
Crianças
Microbiota intestinal
Obesidade
PCR quantitativo

Abstract in Portuguese

O objetivo deste trabalho foi analisar comparativamente alguns microrganismos que compõe a microbiota intestinal endógena de crianças eutróficas (30), com sobrepeso (24) e obesas (30) entre 3 a 11 anos, a partir de amostras fecais. Foi realizado o isolamento de espécies de Bacteroides, Parabacteroides e Clostridium; a identificação de B. fragilis e C. perfringens enterotoxigênicos; e a detecção quantitativa por PCR (SybrGreen) de B. fragilis, B.vulgatus, P. distasonis, C. perfringens, C. difficile, Bifidobacterium spp., Lactobacillus spp., Bacteroidales e Clostridium (cluster I). As espécies C. perfringens e B. vulgatus foram as mais isoladas; nenhum isolado B. fragilis foi enterotoxigênico; todos C. perfringens foram classificados como tipo A e destes 8,7% e 12,2% possuiam os genes tpeL e netB, respectivamente. C. perfringens, C. difficile e Bifidobacterium spp. estavam em maior quantidade em crianças eutróficas, enquanto obesos e com sobrepeso apresentaram maior número de Lactobacillus spp. e Bacteroidales.

Title in English

Evaluation of some microorganism from endogenous intestinal microbiota of normal weight, overweight and obese schoolchildren.

Keywords in English

Bacteroides
Clostridium
Children
Intestinal microbiota
Obesity
Quantitative PCR

Abstract in English

The aim of this study was to evaluate some microorganism from endogenous intestinal microbiota of normal weight (30), overweight (24) and obese (30) children between 3 and 11 years, from fecal samples. It was performed the isolation of species of Bacteroides, Parabacteroides and Clostridium; the identification of B. fragilis and C. perfringens enterotoxigenic; and the quantitative detection by PCR (SybrGreen) B. fragilis, B. vulgatus, P. distasonis, C. perfringens, C. difficile, Bifidobacterium spp., Lactobacillus spp., Bacteroidales and Clostridium (cluster I). The species C. perfringens and B. vulgatus were the most isolated; no isolated B. fragilis was enterotoxigenic; all C. perfringens were classified as type A and these 8.7% and 12.2% harbored tpeL and netB genes, respectively. C. perfringens, C. difficile and Bifidobacterium spp. were in greater quantity in normal weight children while obese and overweight showed a higher number of Lactobacillus spp. and Bacteroidales.

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Publishing Date

2014-08-14

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