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Master's Dissertation
DOI
https://doi.org/10.11606/D.42.2015.tde-10122015-085901
Document
Author
Full name
Diana Carolina Tusso Pinzón
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Gomez, José Gregorio Cabrera (President)
Maldonado, Gabriel Padilla
Ramos, Patrícia Locosque
Title in Portuguese
Caracterização do gene PHA sintase de bactérias isoladas a partir de amostras de solo.
Keywords in Portuguese
Burkholderia sp.
Pseudomonas sp.
Biopolímeros
PHA sintase
Polihidroxialcanoatos
Abstract in Portuguese
Os polihidroxialcanoatos (PHA) são poliésteres bacterianos. Na sua biossíntese, a PHA sintase incorpora monômeros 3HA à cadeia polimérica. O objetivo deste trabalho foi estudar o potencial da PHA sintase de 2 isolados do gênero Burkholderia sp. na produção de copolímeros. Construiram-se linhagens recombinantes que abrigavam os genes da PHA sintase classe I, em mutantes de Pseudomonas sp. e Burkholderia sacchari, que não acumulam PHA. Foram realizados ensaios de acúmulo de PHA usando glicose como fonte de carbono, apresentando a produção de unidades de 3HB, 3HO e 3HD nas linhagens recombinantes de Pseudomonas sp. As linhagens recombinantes de B. sacchari incorporaram como único constituinte P(3HB). Ensaios de acúmulo de PHA foram realizados nas linhagens recombinantes de B. sacchari, usando como co-substratos diferentes ácidos graxos, sendo detectada a incorporação de unidades de 3HV e 3HHx além do 3HB, quando foram fornecidos acido hexanóico e valérico. Estes resultados indicam que as PHA sintases classe I são capazes de incorporar diferentes unidades monoméricas.
Title in English
PHA synthase gene characterization of bacteria isolated from soil samples.
Keywords in English
Burkholderia sp.
Pseudomonas sp.
Biopolymers
PHA synthase
Polhyhydroxyalkanoate
Abstract in English
The polyhydroxyalkanoates (PHA) are bacterial polyester. In their biosynthesis, the PHA synthase incorporates monomers 3HA to the polymer chain. The objective of this work was to study the potential of PHA synthase of 2 isolates of the genus Burkholderia sp. in the production of copolymers. Were constructed recombinant strains that housed the genes of PHA synthase class I mutants of Pseudomonas sp. and Burkholderia sacchari, which do not accumulate PHA. PHA accumulation assays were performed using glucose as carbon source, showing the production of units of 3HB, 3HO and 3HD in recombinant strains of Pseudomonas sp. The recombinant strains of B. sacchari incorporated as single constituent P(3HB). PHA accumulation assays were performed on the recombinant strains of B. sacchari, Using as co-substrates different fatty acids, being detected the incorporation of units of 3HV and 3HHx beyond the 3HB, when were supplied hexanoic acid and valerico. These results indicate that the PHA synthases class I are able to incorporate different monomer units.
 
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Publishing Date
2015-12-10
 
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