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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2015.tde-06102015-190949
Document
Author
Full name
Sabina Moser Tralamazza
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2015
Supervisor
Committee
Correa, Benedito (President)
Furlong, Eliana Badiale
Ishida, Kelly
Pimentel, Ida Chapaval
Sabino, Myrna
Title in Portuguese
Diversidade fúngica, análise polifásica do gênero Fusarium e determinação de desoxinivalenol e zearalenona em grãos de trigo de diferentes regiões do Brasil.
Keywords in Portuguese
Fusarium
Desoxinivalenol
Fungos
Tricotecenos
Trigo
Zearalenona
Abstract in Portuguese
O presente trabalho visou utilizar o perfil polifásico, envolvendo características fenotípicas e genotípicas, na identificação de Fusarium spp. isolados de grãos de trigo como também investigar a presença das principais toxinas: desoxinivalenol e zearalenona nos grãos de trigo de três regiões produtoras de trigo (São Paulo, Paraná e Rio Grande do Sul). Nossos resultados indicaram que os gêneros mais frequentes no trigo foram Alternaria, Fusarium e Epicoccum. A determinação do genótipo por qPCR demonstrou predomínio de 15-ADON seguido por NIV e 3-ADON. A quantificação de DNA demostrou que o perfil 15-ADON foi responsável por 96% de todo DNA quantificado, seguido por NIV com 3.84% e 3-ADON, com somente 0.06%, indicando que 15-ADON, é o principal genótipo de tricoteceno nos grãos de trigo nacional. A toxina desoxinivalenol foi detectada em todas amostras analisadas, com mediana de 323, 466 e 783 µg/kg em SP, PR e RS, respectivamente. A determinação de zearalenona demonstrou contaminação em 100%, 80% e 42% dos grãos dos Estados do RS, PR e SP e medianas de 843, 100 e 14 µg/kg, respectivamente.
Title in English
Fungal diversity, polyphasic analysis of the genus Fusarium and determination of deoxynivalenol and zearalenone in wheat grains from different regions of Brazil.
Keywords in English
Fusarium
Deoxynivalenol
Fungi
Trichothecenes
Wheat
Zearalenone
Abstract in English
The present work aimed to use a polyphasic approach, by phenotypic and genotypic characteristics for the identification of Fusarium strains from wheat grains as well to investigate the presence of deoxynivalenol and zearalenone on wheat grains from three wheat producers States (Parana, Rio Grande do Sul and Sao Paulo). Our results showed that the main genera found were Alternaria, Fusarium and Epicoccum. Genotype detection from qPCR revealed predominance of 15-ADON, followed by NIV and 3-ADON. The qPCR demonstrated that 15-ADON genotype was responsible for 96% of all DNA quantified, followed by NIV with 3.84% and 3-ADON with 0.06%, indicating that 15-ADON is the main trichothecene genotype from Brazilian wheat grains. The toxin deoxynivalenol was detected in all 150 samples analyzed from wheat grains, with medians of 323, 466 and 783 µg/kg in SP, PR and RS, respectively. The zearalenone determination showed contamination on 100%, 80% and 42% of wheat grains from RS, PR and SP State and medians of 843, 100 and 14 µg/kg, respectively.
 
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Publishing Date
2015-10-08
 
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