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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2017.tde-05102017-163314
Document
Author
Full name
Priscila Jane Romano de Oliveira Gonçalves
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2017
Supervisor
Committee
Araujo, Welington Luiz de (President)
Andreote, Fernando Dini
Galhardo, Rodrigo da Silva
Passaglia, Luciane Maria Pereira
Souza, Ana Olívia de
Title in Portuguese
Caracterização e avaliação do papel do gene wcbE de Burkholderia seminalis linhagem TC3.4.2R3 na interação microbiana.
Keywords in Portuguese
Burkholderia seminalis
Cluster wcb
Glicosiltransferase
Interações microbianas
Temperatura
Abstract in Portuguese
Burkholderia seminalis tem sido encontrada tanto em interações patogênicas, quanto não patogênicas. O gene wcbE codifica uma glicosiltransferase e pertence ao cluster wcb, que está relacionado à síntese de cápsula. O objetivo deste trabalho foi investigar o papel do gene wcbE e da temperatura nas interações microbianas de B. seminalis TC3.4.2R3. A produção de biofilme, EPS e compostos antifúngicos foi maior a 28 ºC. Por outro lado, a motilidade, virulência e respostas ao estresse foram maiores a 37 ºC. wcbE produziu menos biofilme que WT e foi atenuada em G. mellonella a 37 ºC, destacando a importância da glicosiltransferase na patogênese. Além disso, wcbE perdeu a habilidade de inibir fungos fitopatogênicos. Embora B. seminalis seja um membro do Bcc, é eficiente contra patógenos clínicos e ambientais, indicando que esta linhagem pode ter interações múltiplas no ambiente. A temperatura e o gene de glicosiltransferase desempenharam um papel crucial nas interações ambientais de B. seminalis TC3.4.2R3.
Title in English
Characterization and evaluation of the role of wcbE gene from Burkholderia seminalis strain TC3.4.2R3 in microbial interaction.
Keywords in English
Burkholderia seminalis
wcb cluster
Glycosyltransferase
Microbial interactions
Temperature
Abstract in English
Burkholderia seminalis has been found in both pathogenic and nonpathogenic interactions. The wcbE gene encodes a glycosyltransferase and belongs to the wcb cluster, which is related to capsule synthesis. The aim of this work was to investigate the role of the wcbE gene and temperature in the microbial interactions of B. seminalis TC3.4.2R3. The production of biofilm, EPS and antifungal compounds was higher at 28 ºC. On the other hand, the motility, virulence and stress responses were higher at 37 ° C. wcbE produced less biofilm than WT and was attenuated in G. mellonella at 37 ° C, highlighting the importance of glycosyltransferase in the pathogenesis. Furthermore, wcbE lost the ability to inhibit phytopathogenic fungi. Although B. seminalis is a member of Bcc, it is effective against clinical and environmental pathogens, indicating that this strain may have multiple interactions in the environment. The temperature and the glycosyltransferase gene played a crucial role in the environmental interactions of B. seminalis TC3.4.2R3.
 
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Publishing Date
2017-10-06
 
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