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Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2009.tde-02022010-100915
Document
Author
Full name
Rodrigo Assunção Moura
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Castro, Antonio Fernando Pestana de (President)
Franzolin, Marcia Regina
Piazza, Roxane Maria Fontes
Takahara, Silvia Yumi Bando
Yano, Tomomasa
Title in Portuguese
Estudo das relações clonais entre amostras de Escherichia coli enteropatogênica atípica de origem animal e humana.
Keywords in Portuguese
Escherichia coli
Diarréia
EPEC atípica
EPEC típica
Infecções bacterianas gram-negativas
MLST
PFGE
Abstract in Portuguese
Quarenta e nove amostras EPEC típica (tEPEC) e atípica (aEPEC) pertencentes a diferentes sorotipos, isoladas de humanos e animais (cães, gatos, bovinos, ovinos, coelhos e sagüis) foram investigadas quanto ao perfil de virulência pela PCR e similaridade clonal por Multilocus Sequence Typing (MLST) e Pulsed-Field Gel Electrophoresis (PFGE). O objetivo deste estudo foi verificar se animais atuam como reservatório e fonte infecção de aEPEC para humanos. Os marcadores de virulência analisados revelaram que cepas aEPEC isoladas de animais possuem potencial para causar diarréia em humanos. As técnicas MLST e PFGE revelaram que amostras isoladas de animais e humanos compartilham relações clonais próximas ou idênticas. Estes resultados indicam que os animais estudados atuam como reservatório de aEPEC e representam fonte de infecção para humanos. Pelo fato de humanos, também atuarem como reservatório de aEPEC, ciclos de infecção cruzada animal-humano não podem ser descartados, pois a dinâmica de transmissão entre reservatórios de aEPEC não é muito bem compreendida.
Title in English
Clonal relationship among atypical enteropathogenic Escherichia coli strains isolated from different animal species and humans.
Keywords in English
Escherichia coli
Atypical EPEC
Bacterial infections Gram-negative
Diarrhea
MLST
PFGE
Typical EPEC
Abstract in English
Forty-nine typical and atypical EPEC strains belonging to different serotypes, isolated from humans, pets (cats and dogs), farm (bovines, sheep and rabbits) and wild animals (monkeys) were investigated for virulence markers and clonal similarity by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The virulence markers analyzed revealed that atypical EPEC strains isolated from animals have the potential to cause diarrhea in humans. Close clonal relationship between human and animal isolates was found with MLST and PFGE. These results indicate that these animals act as atypical EPEC reservoirs and may represent sources of infection for humans. Since humans also act as a reservoir of atypical EPEC strains, the cycle of mutual infection of atypical EPEC between animals and humans, mainly pets and their owners, cannot be ruled out, since the transmission dynamics between the reservoirs are not yet clearly understood.
 
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Publishing Date
2010-03-05
 
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