• JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
  • JoomlaWorks Simple Image Rotator
 
  Bookmark and Share
 
 
Doctoral Thesis
DOI
https://doi.org/10.11606/T.42.2009.tde-02022010-093628
Document
Author
Full name
Larissa Morais Bomilcar do Amaral
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2009
Supervisor
Committee
Stewien, Klaus Eberhard (President)
Barbosa, Maria Luisa
Jerez, José Antonio
Pereira, Luiz Eloy
Richtzenhain, Leonardo José
Title in Portuguese
Epidemiologia e caracterização molecular do Vírus Parainfluenza Humano 1, 2 e 3 em crianças menores de 5 anos de idade atendidas no Hospital Universitário em 2007, São Paulo - Brasil.
Keywords in Portuguese
Epidemiologia
HPIV (Parainfluenza Vírus Humano) tipo 1-2 e 3
Proteínas
Virologia molecular
Vírus
Abstract in Portuguese
O objetivo do presente trabalho é descrever o perfil epidemiológico e molecular dos vírus Parainfluenza em crianças menores de 5 ano de idade, com infecção das vias respiratórias. Por tanto, aspirados de nasofaringe de 742 crianças foram examinadas para os Vírus Parainfluenza Humano(HPIV1, HPIV2 e HPIV3), hRSV, hMPV e IA e IB pela técnica de RT-PCR, durante o ano de 2007. Ao todo foram identificados 52(7%) Parainfluenza vírus, sendo 9(17,3%) HPIV1, 8(15,4%) HPIV2 e 35(67,3%) HPIV3. Destas, 12 (23%) foram casos de coinfecções, sendo 3 (25%) de HPIV3 com VRS, 3 (25%) com MPV e 3 (25%) com HPIV1 e 1(8,33%) com HPIV2; além de 1(8,33%) do HPIV1 com hVSR; e 1(8,33%) de HPIV2 com o hMPV. Com relação ao setor de atendimento, dos 52 pacientes com HPIV, 8(5%) dos casos foram atendidos no PA; 38(8,4%) na ENF; 6(18,2%) na UTI. Obtivemos, no estudo, 21(5,3%) crianças do sexo masculino com infecção por HPIV e 31(9%) de sexo feminino. Quanto a idade, as crianças entre 1 a 23 meses foram as mais frequentemente infectadas. Com relação ao diagnóstico clínico tivemos 19(36,5%) casos com bronquiolites, 13(25%) casos com pneumonia, 7(13,4%) com broncopneumonia, 2(3,9%) de casos de dispnéia e 2(3,9%) de IVAS, além de 9(17,3%) sem informação da sintomatologia. A sazonalidade do HPIV foi marcada pela circulação do HPIV3 durante o ano inteiro, com maior incidência em outubro(primavera). O HPIV 1 e 2 se alternaram durante o ano, onde o HPIV1 circulou no primeiro semestre com maior incidência no mês de abril e maio e o HPIV2 circulou no segundo semestre com maior incidência em outubro, coincidindo com o HPIV3. O Estudo filogenético dos HPIV1 demonstrou 9 mutações nucleotídicas, sendo que 4 são características das amostras brasileiras. Entretanto, quando feito a transdução para aminoácido, apenas uma mutação foi não silenciosa, onde observamos a mudança de glicina, nas amostras do Genbank, para argenina, nas amostras brasileiras, deixando-as em um clado único na topologia da árvore obtida. O HPIV3 apresentou 4 subgrupos distintos durante o ano e suas mutações tanto nucleotídicas quanto de aminoácidos foram todas silenciosas. Em conclusão o vírus parainfluenza representaram a 3ª maior causa de infecção das vias respiratórias inferiores, precedido pelo vírus respiratório Sincicial e o Metapneumovirus.
Title in English
Molecular characterization and epidemiology of Human Parainfluenza Vírus isoled from children below five years old hospitalized at the University Hospital, USP, in 2007, São Paulo - Brazil.
Keywords in English
Epidemiology
HPIV (Human Parainfluenza Viruses) type 1-2 e 3
Molecular virology
Proteins
Virus
Abstract in English
The goal of the present work was to characterize the epidemiologic and molecular profile of the parainfluenza virus in children with less than 5 years of age and presenting respiratory tract infection. Human Parainfluenza (HPIV1, HPIV2, HPIV3), and hRSV, hMPV e IA e IB were evaluated in nasopharyngeal aspirate from 742 children by RT-PCR during the year of 2007. Human parainfluenza was identified in 52(7%) of the samples, which include 9(17,3%) HPIV1, 8(15,4%) HPIV2 and 35(67,3%) HPIV3 17,3% (n=9). Among the 52 cases with parainfluenza, 12 (23%) presented coinfection with other viruses: 3 (25%) coinfection with HPIV3 and VRS, 3 (25%) MPV , 3 (25%) HPIV1 , 1(8,33%) HPIV2 . In addition, it was observed 1(8,33%) of cases with HPIV1 and hVSR; and 1(8,33%) of cases with HPIV2 and hMPV. With respect to the clinical care of the 52 patients with HPIV, 8(5%) were treated at the ER? ; 38(8,4%) at the ENF and 6(18,2%) at the ICU. With respect to gender, a total of 21(5,3%) of the participants with HPIV infections were male and 31(9%) were female. The prevalent age range of participants with infections was 1-23 months of age. The following clinical parameters were observed: bronchiolitis 19(36,5%), pneumonia 13(25%), bronchopneumonia 7(13,4%), dyspnea 2(3,9%) and IVAS 2(3,9%). No clinical information was available for 9(17,3%) cases. HPIV3 infections were detected all year long with an incidence peak in October (Spring). HPIV1 and 2 infections were intercalated during the year. HPIV1 was detected during the first semester with peaks of incidence in April and May whereas HPIV2 was detected during the second semester with peaks of incidence in October, coexisting with HPIV3. HPIV1phylogenetic studies revealed 9 genetic mutations. 4 out of the 9 mutations are exclusively found in the brazilian population. However, only one of these 9 mutations is non-silent and causes a glycine to argenine substitution. When compared to other brazilian sequences in the GenBank, this observation revealed um clado único na topologia da árvore obtida. HPIV3 genotyping included 4 distinct groups detected all year long. All mutations observed in HPIV3 were silent. In conclusion, the parainfluenza virus represent the 3rd major cause of infection of the lower respiratory tract, following the sincicial respiratory virus and the Metapneumovirus.
 
WARNING - Viewing this document is conditioned on your acceptance of the following terms of use:
This document is only for private use for research and teaching activities. Reproduction for commercial use is forbidden. This rights cover the whole data about this document as well as its contents. Any uses or copies of this document in whole or in part must include the author's name.
Publishing Date
2010-03-15
 
WARNING: Learn what derived works are clicking here.
All rights of the thesis/dissertation are from the authors
CeTI-SC/STI
Digital Library of Theses and Dissertations of USP. Copyright © 2001-2024. All rights reserved.