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Master's Dissertation
DOI
10.11606/D.42.2014.tde-14112014-132917
Document
Author
Full name
Marlene Aparecida Ferreira Pinto
Institute/School/College
Knowledge Area
Date of Defense
Published
São Paulo, 2014
Supervisor
Committee
Lotfi, Claudimara Ferini Pacicco (President)
Dale, Camila Squarzoni
Giannella, Maria Lucia Cardillo Correa
Title in Portuguese
Estudo da expressão gênica e proteica do fator de transcrição AP-1 em culturas de células de tumores adrenocorticais.
Keywords in Portuguese
AP-1
Fator de transcrição
Genes JUN e FOS
Tumores adrenocorticais
Abstract in Portuguese
Para compreensão da biologia dos tumores adrenocorticais são utilizados marcadores moleculares que em geral são genes reguladores do ciclo celular. AP-1 é um fator dimérico composto principalmente pelas proteínas JUN (JUN, JUNB e JUND) e FOS. Nesse projeto tivemos como hipótese que as proteínas da família JUN, se correlacionam com proteínas reguladoras do ciclo celular em tumores adrenocorticais, e poderiam ser utilizados no diagnóstico e prognóstico desse tipo de tumor. O objetivo foi analisar o padrão de expressão gênica e proteica (PCR e Immunobloting). A análise por PCR Array em culturas de células de tumores adrenais, mostrou que os genes da família JUN e o gene FOS estão pouco expressos nessas culturas, o que foi confirmado em ensaios de qPCR. Não foi possível determinar um padrão de expressão que diferenciasse os tipos de culturas celulares estudados, ou mesmo tumores adultos e pediátricos. Os tratamentos com ACTH aumentam a expressão da proteina JUN e JUNB, e podem ter certa importância em tumores responsivos à esse hormônio, que merecem análises futuras.
Title in English
Analysis of JUN and FOS gene expression in adult and pediatric adrenocortical tumor cells.
Keywords in English
JUN and FOS genes
Adrenocortical tumors
AP-1
Transcription fator
Abstract in English
In order to have a better comprehension of adrenocortical tumor biology, molecular markers are utillized because they are in general cell cycle regulators. AP-1 is a dimeric factor, compound mainly by JUN (JUN, JUNB, JUND) and FOS proteins. In the present study, our hypothesis is that JUN proteins are correlated with others cell cycle regulatory proteins and could be utilized as a prognose and diagnostic predictor for adrenocortical tumors. To confirm our hypothesi the aim was genic and protein profile analyzes (PCR and Immunobloting). The PCR Array analysis showed that JUN and FOS gene expression were down regulated in these adrenocortical tumors cells which were confirmed through qPCR analysis. The genes expression analysis wasn´t able to stablish a standard of expression between the studied cell cultures or even between adults and pediatric tumors. The ACTH treatments increased JUN and JUNB proteins that may have some significance in responsive tumors to this hormone, and deserve further analysis.
 
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Release Date
2016-11-26
Publishing Date
2014-11-27
 
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